调肝解酒通络方通过调节AMPK信号通路减轻非酒精性脂肪性肝病小鼠的肝脂肪变性。
Tiaogan Jiejiu Tongluo formula alleviates hepatic steatosis in NAFLD mice by regulating AMPK signaling pathway.
作者信息
Lei Yuting, Wang Lianping, Song Linze, Han Jiajun, Ma He, Luo Haoming, Ma Yan, Han Dong
机构信息
College of Pharmacy, Changchun University of Chinese Medicine, Jilin, China.
College of Traditional Chinese Medicine, Jilin Agricultural Science and Technology University, Jilin, China.
出版信息
J Pharm Pharmacol. 2025 Apr 3;77(4):492-500. doi: 10.1093/jpp/rgaf005.
OBJECTIVES
Tiaogan Jiejiu Tongluo formula (TJTF) is a traditional Chinese medicine formula for liver disease. The purpose of this study was to explore the protective effects and mechanisms of TJTF on nonalcoholic fatty liver disease (NAFLD) mice.
METHODS
A NAFLD model of mice was established by a combination of a high-fat diet and CCL4 and then treated with TJTF. The damage to liver tissue was observed through histopathology, and the levels of AST, ALT, TG, TC, LDL-C, and HDL-C in the serum, as well as SOD, GSH, and MDA in the liver tissue were determined using biochemistry or ELISA kit. The expression of proteins related to the AMPK pathway was detected by western blotting.
RESULTS
Biochemical indicators and pathological examination showed that TJTF could enhance the antioxidant capacity of liver tissue and significantly reduce liver lipid deposition. In addition, TJTF significantly increased levels of LKB1 and p-AMPK, and decreased the levels of HMGCR, SREBP-1c, FAS, and ACC.
CONCLUSION
TJTF can alleviate hepatic steatosis and effectively improve NAFLD by regulating AMPK signaling pathway in NAFLD mice.
目的
调肝解酒通络方（TJTF）是一种用于治疗肝病的中药方剂。本研究旨在探讨TJTF对非酒精性脂肪性肝病（NAFLD）小鼠的保护作用及机制。
方法
通过高脂饮食和四氯化碳联合建立NAFLD小鼠模型，然后用TJTF进行治疗。通过组织病理学观察肝组织损伤情况，采用生化法或ELISA试剂盒测定血清中AST、ALT、TG、TC、LDL-C和HDL-C水平以及肝组织中SOD、GSH和MDA水平。通过蛋白质印迹法检测与AMPK通路相关的蛋白质表达。
结果
生化指标和病理检查表明，TJTF可增强肝组织的抗氧化能力，并显著减少肝脏脂质沉积。此外，TJTF显著提高LKB1和p-AMPK水平，降低HMGCR、SREBP-1c、FAS和ACC水平。
结论
TJTF可通过调节NAFLD小鼠的AMPK信号通路减轻肝脏脂肪变性，有效改善NAFLD。
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