Quirion R, Richard J, Dam T V
Brain Res. 1985 May 6;333(2):345-9. doi: 10.1016/0006-8993(85)91590-2.
Levels of serotonin type-2 receptors and choline acetyltransferase (ChAT) were measured in various rat brain regions following unilateral lesions of the nucleus basalis magnocellularis (nBM). As expected, lesions of the nBM markedly decrease cortical ChAT activity. Moreover, [3H]ketanserin (serotonin type-2) binding is significantly decreased in lamina IV of the anterior and middle cortex on the lesioned vs control side. [3H]ketanserin binding in the striatum is not affected by lesions of the nBM. Autoradiograms of [3H]ketanserin binding in lesioned animals show similar results. This suggests that at least a certain proportion of serotonin type-2 receptor binding sites are located on cholinergic terminals in lamina IV of the rat cortex. Therefore, further investigations on cholinergic-serotonergic interactions in the cortex and their possible involvement in senile dementia of the Alzheimer's type appear to be highly relevant.
在大鼠基底前脑大细胞部（nBM）单侧损伤后，检测了其不同脑区中5-羟色胺2型受体和胆碱乙酰转移酶（ChAT）的水平。正如预期的那样，nBM损伤显著降低了皮质ChAT活性。此外，与对照侧相比，损伤侧前皮质和中皮质IV层中[3H]酮色林（5-羟色胺2型）结合显著减少。nBM损伤不影响纹状体中[3H]酮色林结合。损伤动物中[3H]酮色林结合的放射自显影片显示了类似结果。这表明至少一定比例的5-羟色胺2型受体结合位点位于大鼠皮质IV层的胆碱能终末上。因此，对皮质中胆碱能-5-羟色胺能相互作用及其可能参与阿尔茨海默型老年性痴呆的进一步研究似乎具有高度相关性。
