Morrow A L, Loy R, Creese I
Brain Res. 1985 May 20;334(2):309-14. doi: 10.1016/0006-8993(85)90223-9.
Nicotinic cholinergic agonist binding sites were studied in rat hippocampus by the binding of [3H]acetylcholine in the presence of 1.5 microM atropine sulfate. Following transection of the fimbria/fornix there was a 49% increase in the binding of [3H]acetylcholine reflecting an increase in the affinity of the receptor binding site from Kd = 18.82 +/- 3.6 nM in control animals to Kd = 9.06 +/- 1.2 nM in experimental tissue. Chronic administration of the agonist nicotine (4 mg/kg/day) by osmotic minipumps produced an increase in the binding of 10 nM [3H]acetylcholine after 14 days (49% increase over control) and after 28 days (141% increase over controls). These data are consistent with the suggestion that [3H]acetylcholine labels a nicotinic cholinergic receptor in rat brain. Further they support the notion that some of the termination sites of the septal-cholinergic projection to the hippocampus are nicotinic.
在1.5微摩尔硫酸阿托品存在的情况下，通过[3H]乙酰胆碱的结合来研究大鼠海马体中的烟碱型胆碱能激动剂结合位点。切断穹窿/穹窿后，[3H]乙酰胆碱的结合增加了49%，这反映出受体结合位点的亲和力从对照动物中的Kd = 18.82 ± 3.6纳摩尔增加到实验组织中的Kd = 9.06 ± 1.2纳摩尔。通过渗透微型泵慢性给予激动剂尼古丁（4毫克/千克/天），14天后10纳摩尔[3H]乙酰胆碱的结合增加（比对照增加49%），28天后增加（比对照增加141%）。这些数据与[3H]乙酰胆碱标记大鼠脑中烟碱型胆碱能受体的观点一致。此外，它们支持这样一种观点，即从隔区到海马体的胆碱能投射的一些终末位点是烟碱型的。
