Cochran M
Brain Res. 1985 Mar;351(1):89-100. doi: 10.1016/0165-3806(85)90234-2.
Schwann cells derived from mouse or rat dorsal root ganglia (DRG) were co-cultured with either DRG neurons or nerve growth factor (NGF)-responsive PC12 pheochromocytoma cells for up to 7 weeks. When Schwann cells were grown in the presence of DRG neurites, they displayed normal ensheathing behavior and produced basal laminae and small diameter collagen fibrils within 5-19 days in vitro. However, when Schwann cells were co-cultured in direct contact with PC12 cells and without DRG neurons, they largely failed to ensheath PC12 neurites, and failed to assemble either basal lamina or small diameter collagen fibrils at any point during 7 weeks. Schwann cell proliferation continued in the presence of PC12 neurites, indicating that PC12 cells produced a mitogenic activity for Schwann cells functionally similar to previously described neurite-associated activities. These results demonstrate that Schwann cell contact with PC12 cells does not elicit the final morphogenetic events in Schwann cells (ensheathment, basal lamina formation and collagen fibril assembly) that normally occur when Schwann cells are co-cultured in contact with DRG neurons.
将源自小鼠或大鼠背根神经节（DRG）的雪旺细胞与DRG神经元或对神经生长因子（NGF）有反应的PC12嗜铬细胞瘤细胞共培养长达7周。当雪旺细胞在DRG神经突存在的情况下生长时，它们表现出正常的包裹行为，并在体外5 - 19天内产生基底膜和小直径胶原纤维。然而，当雪旺细胞与PC12细胞直接共培养且无DRG神经元时，它们在很大程度上无法包裹PC12神经突，并且在7周内的任何时候都无法组装基底膜或小直径胶原纤维。在PC12神经突存在的情况下，雪旺细胞增殖持续，这表明PC12细胞对雪旺细胞产生了一种促有丝分裂活性，其功能类似于先前描述的与神经突相关的活性。这些结果表明，雪旺细胞与PC12细胞的接触不会引发雪旺细胞中最终的形态发生事件（包裹、基底膜形成和胶原纤维组装），而这些事件在雪旺细胞与DRG神经元共培养接触时通常会发生。
