Keefer D, Holderegger C
Brain Res. 1985 Apr;351(2):183-94. doi: 10.1016/0165-3806(85)90190-7.
This study examines the prenatal and neonatal development of estrogen receptors in the central nervous system of the mouse. [3H]Diethylstilbestrol (DES) was injected into pregnant mice on days 4, 7, 10, 13, 14, 15 and 17 of gestation or into neonates. DES is an estrogen agonist that circumvents the alpha-fetoprotein barrier, thereby gaining access to intracellular estrogen receptors. Sixty minutes after injection whole embryos, fetuses or neonates were rapidly frozen and processed for autoradiography. Although the transplacental movement of the isotope was confirmed in all age groups evidence for nuclear estrogen receptors was not seen in the brain until day E14. On this day a few labeled cells first appeared in the basal hypothalamus, preoptic area, amygdala, midbrain and spinal cord. The number and the labeling intensity of target cells increased in each of these areas on days E15, E17 and P0. The first appearance of estrogen receptors closely follows the reported birthdates of neurons in these regions.
本研究考察了小鼠中枢神经系统中雌激素受体的产前及新生儿期发育情况。在妊娠第4、7、10、13、14、15和17天，将[3H]己烯雌酚（DES）注射到怀孕小鼠体内，或注射到新生小鼠体内。DES是一种雌激素激动剂，可绕过甲胎蛋白屏障，从而进入细胞内雌激素受体。注射60分钟后，将整个胚胎、胎儿或新生儿迅速冷冻，并进行放射自显影处理。尽管在所有年龄组中均证实了同位素的经胎盘移动，但直到胚胎第14天，在脑中才观察到核雌激素受体的证据。在这一天，少数标记细胞首次出现在下丘脑基部、视前区、杏仁核、中脑和脊髓。在胚胎第15、17天和出生后第0天，这些区域中靶细胞的数量和标记强度均有所增加。雌激素受体的首次出现与这些区域中报道的神经元出生日期密切相关。
