Huang Xiaokai, Lu Jinyu, Deng Changmi, Tang Weixian, Wang Xilin, Zhou Haixia, Zhang Jiao, Cheng Jiwen, Li Suhong, He Jing, Ruan Jichen
Department of Hematology, The Second Affiliated Hospital, Yuying Children's Hospital of Wenzhou Medical University, 109 West Xueyuan Road, Wenzhou, 325027, Zhejiang, China.
The Key Laboratory of Pediatric Hematology and oncology Diseases of Wenzhou, the Second Affiliated Hospital, Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, China.
BMC Cancer. 2025 Feb 14;25(1):260. doi: 10.1186/s12885-025-13670-7.
Wilms tumor is among the most common pediatric malignant tumors. Although mA modification influences the structure and function of RNA and participates in tumorigenesis, the relationship between mA methyltransferase TRMT61B gene polymorphisms and Wilms tumor susceptibility is unclear.
We examined the relationship between TRMT61B gene rs4563180 G > C polymorphism (detected by TaqMan probe method) in 414 children with Wilms tumor and 1199 healthy controls. The relationship between the genotype of each sublayer and the risk of Wilms tumor was studied by stratified analysis. The GTEx database was used to analyze the influence of TRMT61B rs4563180 G > C polymorphism on mRNA expression.
The TRMT61B gene polymorphism significantly reduced the susceptibility to Wilms tumor (GC vs. GG: adjusted odds ratio [AOR] = 0.72, 95% confidence interval [CI] = 0.56-0.93, P = 0.012; GC/CC vs. GG: AOR = 0.76, 95% CI = 0.60-0.96, P = 0.021). GC/CC genotype had a protective effect in boys and children with stage III tumors compared with rs4563180 GG genotype. Additionally, the C allele was significantly associated with decreased mRNA expression of TRMT61B gene compared with rs4563180G allele in cultured fibroblasts (P = 3.3e - 80), EBV-transformed lymphocytes (P = 9.5e - 14), and whole blood (P = 6.0e - 12).
Our results confirm that TRMT61B gene is associated with the development of Wilms tumors, but its underlying mechanism requires further exploration.
肾母细胞瘤是最常见的儿童恶性肿瘤之一。虽然 mA 修饰影响 RNA 的结构和功能并参与肿瘤发生，但 mA 甲基转移酶 TRMT61B 基因多态性与肾母细胞瘤易感性之间的关系尚不清楚。
我们检测了 414 例肾母细胞瘤患儿和 1199 例健康对照者中 TRMT61B 基因 rs4563180 G＞C 多态性（采用 TaqMan 探针法检测）。通过分层分析研究各亚组基因型与肾母细胞瘤风险之间的关系。利用 GTEx 数据库分析 TRMT61B rs4563180 G＞C 多态性对 mRNA 表达的影响。
TRMT61B 基因多态性显著降低了肾母细胞瘤的易感性（GC 与 GG 相比：调整后的优势比[AOR] = 0.72，95%置信区间[CI] = 0.56 - 0.93，P = 0.012；GC/CC 与 GG 相比：AOR = 0.76，95%CI = 0.60 - 0.96，P = 0.021）。与 rs4563180 GG 基因型相比，GC/CC 基因型在男孩和 III 期肿瘤患儿中具有保护作用。此外，与 rs4563180 G 等位基因相比，C 等位基因在培养的成纤维细胞（P = 3.3e - 80）、EBV 转化的淋巴细胞（P = 9.5e - 14）和全血（P = 6.0e - 12）中与 TRMT61B 基因 mRNA 表达降低显著相关。
我们的结果证实 TRMT61B 基因与肾母细胞瘤的发生有关，但其潜在机制需要进一步探索。
