Long Yunfei, Chen Xin, Chen Jia, Zhang Haoran, Lin Ying, Cheng Shuyuan, Pu Neng, Zhou Xuandong, Sheng Renzhi, Abubakar Yakubu Saddeeq, Zheng Huawei, Yun Yingzi, Lu Guodong, Wang Zonghua, Zheng Wenhui
State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Key Laboratory of Bio-pesticide and Chemistry Biology, Ministry of Education, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350000, China.
New Phytol. 2025 Apr;246(2):666-688. doi: 10.1111/nph.70006. Epub 2025 Feb 15.
In eukaryotes, the retromer complex plays a crucial role in the sorting and retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN). Despite its importance, the molecular details of this intracellular transport process remain unclear. Here, we have identified a Golgi-associated retrograde protein (GARP) complex as a mediator of vesicle transport that facilitates the recruitment of the retromer complex to the TGN to exert its functions. The GARP complex is mainly localized in the TGN where it interacts with the retromer complex. This interaction is evolutionarily conserved across species. Furthermore, we identified FgKex2 and FgSnc1 as cargo proteins in the GARP/retromer-mediated recycling pathway. Loss of GARP or retromer results in a complete missorting of FgKex2 and FgSnc1 into the vacuolar degradation pathway, which affects the growth, development, biogenesis of toxisomes and pathogenicity of Fusarium graminearum. In summary, we demonstrate for the first time that GARP promotes the recruitment of retromer from endosomes to the TGN, thereby establishing a GARP/retromer transport pathway that coordinates the recycling of cargo proteins FgKex2 and FgSnc1. This process is essential for maintaining sustained growth and development and significantly contributes to the pathogenicity of F. graminearum.
在真核生物中，回收体复合物在货物蛋白从内体到反式高尔基体网络（TGN）的分选和逆向运输中起关键作用。尽管其很重要，但这种细胞内运输过程的分子细节仍不清楚。在这里，我们已确定一种高尔基体相关逆向蛋白（GARP）复合物作为囊泡运输的介质，它促进回收体复合物募集到TGN以发挥其功能。GARP复合物主要定位于TGN，在那里它与回收体复合物相互作用。这种相互作用在物种间具有进化保守性。此外，我们确定FgKex2和FgSnc1是GARP/回收体介导的再循环途径中的货物蛋白。GARP或回收体的缺失导致FgKex2和FgSnc1完全错分到液泡降解途径，这影响了禾谷镰刀菌的生长、发育、毒素体生物合成及致病性。总之，我们首次证明GARP促进回收体从内体募集到TGN，从而建立了一个协调货物蛋白FgKex2和FgSnc1再循环的GARP/回收体运输途径。这一过程对于维持持续的生长和发育至关重要，并显著促进了禾谷镰刀菌的致病性。
