一种从人白细胞中提取和纯化DNA的快速方法。
A rapid method for the extraction and purification of DNA from human leukocytes.
作者信息
Potter A A, Hanham A F, Nestmann E R
出版信息
Cancer Lett. 1985 Apr;26(3):335-41. doi: 10.1016/0304-3835(85)90058-8.
A rapid method for the extraction and purification of DNA from human leukocytes was developed. Crude nucleic acids were obtained by sodium dodecylsulfate (SDS) lysis and potassium acetate precipitation of other cellular material, and the DNA was purified by ribonuclease digestion, diethylaminoethyl (DEAE) cellulose chromatography and ethanol precipitation. DNA obtained by this method is biologically active as reflected by its ability to act as substrate for various nucleases and T4 DNA ligase. The yield was sufficiently high that DNA from less than 1 ml of blood could be used for a number of reactions.
开发了一种从人白细胞中提取和纯化DNA的快速方法。通过十二烷基硫酸钠（SDS）裂解和醋酸钾沉淀其他细胞物质获得粗核酸，然后通过核糖核酸酶消化、二乙氨基乙基（DEAE）纤维素色谱法和乙醇沉淀对DNA进行纯化。通过这种方法获得的DNA具有生物活性，这体现在它能够作为各种核酸酶和T4 DNA连接酶的底物。产量足够高，以至于从不到1毫升血液中提取的DNA可用于多种反应。
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