Wan Yu, Pike Rachel, Harley Alessandra, Mumin Zaynab, Potterill Isabelle, Meunier Danièle, Ganner Mark, Getino Maria, Coelho Juliana, Jauneikaite Elita, Moganeradj Kartyk, Brown Colin S, Holmes Alison H, Demirjian Alicia, Hopkins Katie L, Pichon Bruno
HCAI, Fungal, AMR, AMU and Sepsis Division, UK Health Security Agency, London, United Kingdom.
NIHR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, Department of Infectious Disease, Imperial College London, London, United Kingdom.
BMC Genom Data. 2025 Feb 15;26(1):12. doi: 10.1186/s12863-025-01303-8.
Staphylococcus capitis is part of the human microbiome and an opportunistic pathogen known to cause catheter-associated bacteraemia, prosthetic joint infections, skin and wound infections, among others. Detection of S. capitis in normally sterile body sites saw an increase over the last decade in England, where a multidrug-resistant clone, NRCS-A, was widely identified in blood samples from infants in neonatal intensive care units. To address a lack of complete genomes and antibiograms of S. capitis in public databases, we performed long- and short-read whole-genome sequencing, hybrid genome assembly, and antimicrobial susceptibility testing of 22 diverse isolates.
We present complete genome assemblies of two S. capitis type strains (subspecies capitis: DSM 20326; subspecies urealyticus: DSM 6717) and 20 clinical isolates (NRCS-A: 10) from England. Each genome is accompanied by minimum inhibitory concentrations of 13 antimicrobials including vancomycin, teicoplanin, daptomycin, linezolid, and clindamycin. These 22 genomes were 2.4-2.7 Mbp in length and had a GC content of 33%. Plasmids were identified in 20 isolates. Resistance to teicoplanin, daptomycin, gentamicin, fusidic acid, rifampicin, ciprofloxacin, clindamycin, and erythromycin was seen in 1-10 isolates. Our data are a resource for future studies on genomics, evolution, and antimicrobial resistance of S. capitis.
头状葡萄球菌是人类微生物群的一部分，也是一种机会致病菌，已知可引起导管相关菌血症、人工关节感染、皮肤和伤口感染等。在过去十年中，英格兰在通常无菌的身体部位检测到头状葡萄球菌的情况有所增加，在新生儿重症监护病房的婴儿血液样本中广泛发现了一种多重耐药克隆株NRCS-A。为了解决公共数据库中头状葡萄球菌完整基因组和抗菌谱缺乏的问题，我们对22株不同的菌株进行了长读长和短读长全基因组测序、混合基因组组装以及抗菌药敏试验。
我们展示了两株头状葡萄球菌标准菌株（头状亚种：DSM 20326；解脲亚种：DSM 6717）以及来自英格兰的20株临床分离株（NRCS-A：10株）的完整基因组组装结果。每个基因组都附有13种抗菌药物的最低抑菌浓度，包括万古霉素、替考拉宁、达托霉素、利奈唑胺和克林霉素。这22个基因组长度为2.4 - 2.7 Mbp，GC含量为33%。在20株分离株中鉴定出了质粒。1 - 10株分离株对替考拉宁、达托霉素、庆大霉素、夫西地酸、利福平、环丙沙星、克林霉素和红霉素存在耐药性。我们的数据为未来关于头状葡萄球菌的基因组学、进化和抗菌耐药性研究提供了资源。
