在新载体萘甲酰-琼脂糖上对大肠杆菌转移RNA进行色谱分离。
Chromatographic fractionation of Escherichia coli transfer RNA on a new support, naphthoyl-Sepharose.
作者信息
Hjertén S, Hellman U, Svensson I, Rosengren J
出版信息
J Biochem Biophys Methods. 1979 Oct;1(5):263-73. doi: 10.1016/0165-022x(79)90001-0.
PMID:399613
Abstract
The noncharged naphthoyl-Sepharose CL-6B has been prepared. Escherichia coli tRNA binds to this new adsorbent in 0.75 M ammonium sulphate at neutral pH at room temperature. Using a negative salt gradient, the tRNAs are eluted in a defined order. The chromatographic pattern is clearly different from those of other commonly used tRNA separation techniques.
摘要
已制备出不带电荷的萘甲酰-琼脂糖凝胶CL-6B。在室温中性pH条件下，大肠杆菌转运核糖核酸（tRNA）在0.75M硫酸铵中与这种新的吸附剂结合。使用负盐梯度，tRNA按特定顺序洗脱。该色谱图谱与其他常用的tRNA分离技术的图谱明显不同。
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