Kojima N, Iida J, Araki Y, Ito E
Eur J Biochem. 1985 Jun 3;149(2):331-6. doi: 10.1111/j.1432-1033.1985.tb08930.x.
Structural studies were carried out on the polymer chains and their linkage regions in two kinds of teichoic acids, poly(N-acetylglucosamine 1-phosphate) [poly(GlcNAc-1-P)] and glycerol teichoic acid, bound to peptidoglycan in the cell walls of Bacillus pumilus AHU 1650. The poly(GlcNAc-1-P)-glycan complex isolated from lysozyme digests of the cell walls contained mannosamine and glycerol as minor components. On the basis of proton NMR spectroscopic data and isolation of N-acetylglucosamine 4-phosphate from acid hydrolysates, the poly(GlcNAc-1-P) was shown to be a polymer in which N-acetylglucosamine 1-phosphate units are joined at C-4 of the glucosamine residues. Mild alkaline hydrolysis of the poly(GlcNAc-1-P)-glycan complex gave a mannosamine-linked glycan fragment and the acidic polymer fraction that contained glycerol residues. Mild acid treatment of the mannosamine-linked glycan fragment gave the linkage disaccharide, ManNAc(beta 1----4)GlcNAc, whereas the acidic polymer fraction was degraded by this treatment into N-acetylglucosamine 4-phosphate and a glycerol-containing fragment characterized as P-(Gro-P)7 (Gro = glycerol). On the other hand, direct mild acid hydrolysis of the complex gave a fragment characterized as P-(Gro-P)7-ManNAc(beta 1----4)GlcNAc. These results lead to a conclusion that in the cell walls the poly(GlcNAc-1-P) chain is attached to peptidoglycan through a linkage unit, (Gro-P)7-ManNAc(beta 1----4)GlcNAc. By means of similar procedures, it was shown that the other cell wall polymer, glycerol teichoic acid, is also attached to peptidoglycan through the same disaccharide, ManNAc(beta 1----4)GlcNAc.
对短小芽孢杆菌AHU 1650细胞壁中与肽聚糖结合的两种磷壁酸，即聚（N-乙酰葡糖胺1-磷酸）[聚（GlcNAc-1-P）]和甘油磷壁酸中的聚合物链及其连接区域进行了结构研究。从细胞壁的溶菌酶消化物中分离出的聚（GlcNAc-1-P）-聚糖复合物含有甘露糖胺和甘油作为次要成分。根据质子核磁共振光谱数据以及从酸水解物中分离出N-乙酰葡糖胺4-磷酸，表明聚（GlcNAc-1-P）是一种聚合物，其中N-乙酰葡糖胺1-磷酸单元在葡糖胺残基的C-4处连接。聚（GlcNAc-1-P）-聚糖复合物的温和碱性水解产生了一个与甘露糖胺相连的聚糖片段和含有甘油残基的酸性聚合物部分。对与甘露糖胺相连的聚糖片段进行温和酸处理得到连接二糖，ManNAc(β1→4)GlcNAc，而酸性聚合物部分经此处理降解为N-乙酰葡糖胺4-磷酸和一个表征为P-(Gro-P)7（Gro =甘油）的含甘油片段。另一方面，复合物的直接温和酸水解产生了一个表征为P-(Gro-P)7-ManNAc(β1→4)GlcNAc的片段。这些结果得出一个结论，即在细胞壁中聚（GlcNAc-1-P）链通过连接单元（Gro-P）7-ManNAc(β1→4)GlcNAc连接到肽聚糖上。通过类似的程序表明，另一种细胞壁聚合物甘油磷壁酸也通过相同的二糖ManNAc(β1→4)GlcNAc连接到肽聚糖上。
