通过高效液相色谱法纯化肝脏和肝癌细胞膜蛋白。
Purification of liver and hepatoma membrane proteins by high-performance liquid chromatography.
作者信息
Josić D, Schuett W, Neumeier R, Reutter W
出版信息
FEBS Lett. 1985 Jun 3;185(1):182-6. doi: 10.1016/0014-5793(85)80766-3.
PMID:3996595
Abstract
This paper documents the recovery of selected proteins from hepatic plasma membranes. Initial purification, achieved by a series of stepwise extractions, facilitates the subsequent purification by HPLC. Examples are provided to illustrate the recovery of specific proteins from two Morris hepatoma lines and the liver.
摘要
本文记录了从肝细胞膜中回收特定蛋白质的过程。通过一系列逐步提取实现的初步纯化，有助于后续通过高效液相色谱法（HPLC）进行纯化。文中提供了实例，以说明从两种莫里斯肝癌细胞系和肝脏中回收特定蛋白质的情况。
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