Identification and exploration of anticancer activity of novel peptides isolated from the edible bivalve in hepatic and colon cancer cell lines.

BACKGROUND

The requirement for relevant and safe drugs for cancer treatment is considered a challenge. Recently, marine isolated compounds with various therapeutic targets have attracted many researchers.

AIM

Isolation and identification of potential anticancer peptides from edible soft tissues.

METHODOLOGY

specimens were collected and peptides were extracted, purified with FPLC, and tested on normal (hepatocytes and VERO) and cancer (HepG2, and HT-29) cells. Bioactive fractions were tested by tandem mass spectrometry.

RESULTS

Five different fractions were purified according to ionic charges and two fractions (4 and 5) showed a potent anticancer activity with a total anticancer score threshold of ≥ 0.5, and hydrophilicity mean of 1.75 that related to stability and solubility. The apoptotic and autophagy-related markers were significantly up-regulated in both HepG2 and HT-29 cells treated with IC of bioactive peptides' fractions 4 and 5, explaining their underlying mechanism of action.

CONCLUSION

Natural source peptides derived from the soft tissue of could be exploited for the treatment of cancers and a deep in silico study will be performed for further investigation and deep function identification.