Chen Chen, Song Hong, Yang Lina, Wang Shengnan, Li Jinxin, Han Lu, Fan Hongliang, Liu He
College of Food Science and Technology, Bohai University, Jinzhou 121013, China.
College of Food Science and Technology, Bohai University, Jinzhou 121013, China.
Int J Biol Macromol. 2025 May;305(Pt 2):141123. doi: 10.1016/j.ijbiomac.2025.141123. Epub 2025 Feb 17.
Soybean hull polysaccharide (ASP), a pectic polysaccharide with potential effects on regulating lipid metabolism through gut microbiota and their metabolites, was studied to explore its regulatory mechanisms. The study examined the alleviating effect of ASP on high-fat diet (HFD) zebrafish through histopathological examination and biochemical analysis. Additionally, the lipid-lowering mechanism was analyzed combined with metabolomics and the proliferation of gut microbiota. Results showed that ASP significantly ameliorated body weight and blood lipids and also reduced hepatic lipid accumulation and hepatic lipid peroxidation damage. Bioinformatics analyses showed that ASP administration (240 μg/day) generated significant gut microbiota structural changes in high-fat diet-fed zebrafish, in particular, reducing Proteobacteria and increasing Cetobacterium relative abundance levels. ASP upregulates propionic acid, linoleic acid, 6-hydroxyhexanoic acid, and l-threonine biosynthesis by mediating gut microbiota regulation of amino acid and lipid metabolic pathways. Utilizing a correlation network derived from Spearman's correlation coefficients, significant microbial phylotypes that react to ASP demonstrated a strong association with parameters linked to lipid metabolic disorders. Fecal metabolites (e.g. propionic acid) were positively correlated with gut microbiota (e.g. Cetobacterium) in the high-dose ASP group. Our results provide a theoretical basis for new ASP prebiotic formulations to target the gut microbiota and prevent lipid metabolic disorders.
大豆壳多糖（ASP）是一种果胶多糖，可能通过肠道微生物群及其代谢产物对脂质代谢产生调节作用，本研究旨在探索其调节机制。该研究通过组织病理学检查和生化分析，考察了ASP对高脂饮食（HFD）斑马鱼的缓解作用。此外，结合代谢组学和肠道微生物群增殖情况分析了其降脂机制。结果表明，ASP显著改善了体重和血脂，还减少了肝脏脂质积累和肝脏脂质过氧化损伤。生物信息学分析表明，给予ASP（240μg/天）可使高脂饮食喂养的斑马鱼肠道微生物群结构发生显著变化，特别是降低变形菌门的相对丰度，增加鲸杆菌属的相对丰度。ASP通过介导肠道微生物群对氨基酸和脂质代谢途径的调节，上调丙酸、亚油酸、6-羟基己酸和L-苏氨酸的生物合成。利用基于斯皮尔曼相关系数的关联网络，对ASP有反应的重要微生物系统型与脂质代谢紊乱相关参数显示出强关联。在高剂量ASP组中，粪便代谢产物（如丙酸）与肠道微生物群（如鲸杆菌属）呈正相关。我们的研究结果为新型ASP益生元制剂靶向肠道微生物群和预防脂质代谢紊乱提供了理论依据。
