Guo Zhengdong, Zhu Hongming, Zhang Renmin, Shan Qiang, Wang Yajing
Xuyi People's Hospital.
School of Statistics, Renmin University of China, Beijing, China.
Crit Rev Immunol. 2025;45(2):25-38. doi: 10.1615/CritRevImmunol.2024055296.
Nasopharyngeal carcinoma (NPC), a malignant tumor originating from the epithelium and glands. MicroRNAs (miRNAs) play an essential role in the tumorigenesis and metastasis of NPC. They are effective biomarkers in the detection of malignant progression of NPC. In this study, we analyzed the expression profiles of miRNAs in NPC patients with Gene Expression Omnibus (GEO) database. ceRNA networks of NPC were constructed and target miRNAs were screened. MTT, colony formation and Transwell experiments were used to explore the effects of miR-205-5p on the proliferation, migration and invasion ability of NPC cells. Bioinformatics analysis combined with Double luciferase experiment verified the binding relationship between miR-205-5p and CALM1. We identified 34 long non-coding RNAs (lncRNAs), 22 miRNAs, and 145 messenger RNAs (mRNAs) and constructed a competing endogenous RNAs (ceRNA) network to explain the relationship between RNA expression profiles and NPC progression. Of which, we found that 5 miRNAs (hsa-let-7d-5p, hsa-let-7e-5p, hsa-let-7f-5p, hsa-miR-143-3p and hsa-miR-205-5p) are related to clinical features. We further found that miR-205-5p was highly expressed in NPC cell lines. In addition, MTT, colony formation and Transwell assays showed that miR-205-5p promoted the proliferation, migration and invasion of NPC cells. Double luciferase detection showed that miR-205-5p could target combined with CALM1. In addition, we found that miR-205-5p could promote the proliferation, migration and invasion of NPC cells by inhibited the expression of CALM1. Overall, the present study demonstrated that as a carcinogenic factor, miR-205-5p can affect the malignant progression of NPC by mediating CALM1.
鼻咽癌（NPC）是一种起源于上皮和腺体的恶性肿瘤。微小RNA（miRNA）在鼻咽癌的发生和转移中起重要作用。它们是检测鼻咽癌恶性进展的有效生物标志物。在本研究中，我们利用基因表达综合数据库（GEO）分析了鼻咽癌患者中miRNA的表达谱。构建了鼻咽癌的ceRNA网络并筛选了靶miRNA。采用MTT、集落形成和Transwell实验探讨miR-205-5p对鼻咽癌细胞增殖、迁移和侵袭能力的影响。生物信息学分析结合双荧光素酶实验验证了miR-205-5p与CALM1之间的结合关系。我们鉴定出34个长链非编码RNA（lncRNA）、22个miRNA和145个信使RNA（mRNA），并构建了一个竞争性内源RNA（ceRNA）网络来解释RNA表达谱与鼻咽癌进展之间的关系。其中，我们发现5个miRNA（hsa-let-7d-5p、hsa-let-7e-5p、hsa-let-7f-5p、hsa-miR-143-3p和hsa-miR-205-5p）与临床特征相关。我们进一步发现miR-205-5p在鼻咽癌细胞系中高表达。此外，MTT、集落形成和Transwell实验表明，miR-205-5p促进了鼻咽癌细胞的增殖、迁移和侵袭。双荧光素酶检测显示miR-205-5p可靶向结合CALM1。此外，我们发现miR-205-5p可通过抑制CALM1的表达来促进鼻咽癌细胞的增殖、迁移和侵袭。总体而言，本研究表明，作为一种致癌因子，miR-205-5p可通过介导CALM1影响鼻咽癌的恶性进展。
