Serray Bahija, Sobh Mohammed, Timinouni Mohammed, El Azhari Mohamed
Laboratory of Microbiology, Pharmacology, Biotechnology, and Environment, Faculty of Sciences Ain-Chock, Casablanca, Morocco.
Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.
J Infect Dev Ctries. 2025 Jan 31;19(1):34-39. doi: 10.3855/jidc.20319.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community infections. These infections are becoming increasingly difficult to combat, because of emerging resistance to all classes of antibiotics. Panton-Valentine leukocidin (PVL) is an important virulence factor in MRSA and causes white blood cell destruction, necrosis, and accelerated apoptosis. The aim of this study was to determine the frequency of pvl-positive MRSA in a pediatric hospital, in Marrakech, Morocco.
53 isolates of MRSA were recovered in the hospital from December 2010 to May 2014, and confirmed with biochemical tests (coagulase, mannitol fermentation, and DNase). Then, polymerase chain reaction (PCR) was used to detect pvl.
Among the 259 Staphylococcus aureus isolates collected from various clinical specimens, 53 were identified as MRSA; and the presence of the PVL gene was investigated in them using PCR analysis. Out of the 53 MRSA isolates, only 1 (1.89%) was positive for pvl This pvl-positive MRSA isolate was characterized as staphylococcal cassette chromosome mec IV (SCCmec IV), a type commonly associated with community-acquired MRSA infections.
The study revealed a relatively low prevalence of PVL-positive MRSA among pediatric patients at the University Hospital Center CHU Mohamed VI in Marrakech, with only 1.89% of MRSA isolates testing positive for pvl. Despite this low prevalence, the presence of PVL-positive strains accentuates a potential risk for severe infections in vulnerable children. These findings underscore the imperative need for sustained surveillance and rigorous infection control measures to mitigate the spread of MRSA and other resistant pathogens.
耐甲氧西林金黄色葡萄球菌（MRSA）是医院感染和社区感染的主要原因。由于对各类抗生素的耐药性不断出现，这些感染越来越难以对抗。杀白细胞素（PVL）是MRSA中的一种重要毒力因子，可导致白细胞破坏、坏死和加速细胞凋亡。本研究的目的是确定摩洛哥马拉喀什一家儿科医院中pvl阳性MRSA的频率。
2010年12月至2014年5月期间，从该医院分离出53株MRSA，并通过生化试验（凝固酶、甘露醇发酵和DNA酶）进行确认。然后，使用聚合酶链反应（PCR）检测pvl。
在从各种临床标本中收集的259株金黄色葡萄球菌分离株中，有53株被鉴定为MRSA；并使用PCR分析对其中的PVL基因存在情况进行了研究。在53株MRSA分离株中，只有1株（1.89%）pvl呈阳性。这株pvl阳性MRSA分离株被鉴定为葡萄球菌盒式染色体mec IV（SCCmec IV），这是一种通常与社区获得性MRSA感染相关的类型。
该研究显示，马拉喀什穆罕默德六世大学医院中心的儿科患者中，PVL阳性MRSA的患病率相对较低，只有1.89%的MRSA分离株pvl检测呈阳性。尽管患病率较低，但PVL阳性菌株的存在加剧了弱势儿童发生严重感染的潜在风险。这些发现强调了持续监测和严格感染控制措施对于减轻MRSA和其他耐药病原体传播的迫切需要。
