Norioka N, Okada T, Hamazume Y, Mega T, Ikenaka T
J Biochem. 1985 Jan;97(1):19-28. doi: 10.1093/oxfordjournals.jbchem.a135044.
The amino acid sequence of hen egg yolk-riboflavin binding protein (yolk-RBP) was determined by conventional methods. The sequence was identical with that of hen egg white-riboflavin binding protein except that their carboxyltermini were different, that of yolk-RBP lacked 11 or 13 amino acid residues, while hen plasma-RBP had the same C-terminal sequence as white-RBP. This indicated that the C-terminal 11 or 13 amino acid residues in plasma-RBP might be cleaved off during the incorporation from the blood into the oocyte or in the yolk fluid. Yolk-RBP had the same characteristics as white-RBP, such as N-terminal pyroglutamic acid, polymorphism in the amino acid sequence (Lys/Asn) at the fourteenth residue from the N-terminal end, carbohydrate chains attached to both Asn(36) and Asn(147) residues, and phosphate groups bound to some serine residues in the sequence of Ser(185) to Ser(197) as a cluster. These results led us to the conclusion that yolk- and white-RBPs are bio-synthesized from the same gene in the different organs (liver and oviduct). The carbohydrate composition of yolk-RBP was identical to that of plasma-RBP but different from that of white-RBP showing that the processing of the carbohydrate chains in the liver was different from that in the oviduct.
通过常规方法测定了鸡蛋蛋黄核黄素结合蛋白（蛋黄-RBP）的氨基酸序列。该序列与鸡蛋白核黄素结合蛋白的序列相同，只是它们的羧基末端不同，蛋黄-RBP的羧基末端缺少11或13个氨基酸残基，而鸡血浆-RBP的C末端序列与蛋白-RBP相同。这表明血浆-RBP中的C末端11或13个氨基酸残基可能在从血液掺入卵母细胞或卵黄液的过程中被切除。蛋黄-RBP与蛋白-RBP具有相同的特征，如N末端焦谷氨酸、N末端第14个残基的氨基酸序列（Lys/Asn）多态性、与Asn(36)和Asn(147)残基连接的糖链，以及在Ser(185)至Ser(197)序列中与一些丝氨酸残基结合的磷酸基团簇。这些结果使我们得出结论，蛋黄-RBP和蛋白-RBP是由不同器官（肝脏和输卵管）中的同一基因生物合成的。蛋黄-RBP的碳水化合物组成与血浆-RBP相同，但与蛋白-RBP不同，这表明肝脏中糖链的加工与输卵管中的不同。
