商业dATP样品被ATP污染，导致在分离的HeLa细胞核中进行DNA复制研究时产生错误结果。

Contamination by ATP of commercial dATP samples causing erroneous results in studies of DNA replication in isolated HeLa cell nuclei.

作者信息

Hanaoka F, Takahashi M, Enomoto T, Yamada M, Tanaka S, Wataya Y, Hayatsu H

出版信息

J Biochem. 1985 Jan;97(1):383-6. doi: 10.1093/oxfordjournals.jbchem.a135065.

DOI:10.1093/oxfordjournals.jbchem.a135065

Abstract

dATP at high concentrations was capable of replacing ATP required in the synthesis of Okazaki pieces in isolated HeLa cell nuclei. In addition, the levels of synthesis of high molecular weight DNA were observed to vary depending on the lot of dATP used. Analysis by HPLC revealed that dATP samples of a particular source contained ATP in the range of 0.25-0.43 mol%. With ATP-free dATP, almost no synthesis of high molecular weight DNA was observed, while with impure dATP, a small but significant amount of high molecular weight DNA was synthesized. While this observation confirmed our previous finding that dATP can replace ATP in the synthesis of Okazaki pieces but not in the joining of the pieces, it is also a warning to users of commercial dATP in biochemical and biological studies.

摘要

高浓度的脱氧三磷酸腺苷（dATP）能够替代在分离的海拉细胞核中合成冈崎片段所需的三磷酸腺苷（ATP）。此外，观察到高分子量DNA的合成水平会因所使用的dATP批次不同而有所变化。通过高效液相色谱（HPLC）分析发现，特定来源的dATP样品中ATP的含量在0.25 - 0.43摩尔百分比范围内。使用无ATP的dATP时，几乎未观察到高分子量DNA的合成，而使用不纯的dATP时，则合成了少量但显著量的高分子量DNA。虽然这一观察结果证实了我们之前的发现，即dATP可以在冈崎片段的合成中替代ATP，但在片段连接中则不能，不过这也给生化和生物学研究中商业dATP的使用者敲响了警钟。