DNA合成的ATP类似物在分离细胞核中的作用。

Effect of ATP analogs of DNA synthesis in isolated nuclei.

作者信息

Hershey H V

出版信息

Biochim Biophys Acta. 1977 Dec 2;479(3):256-64. doi: 10.1016/0005-2787(77)90108-3.

Abstract

Optimal synthesis of DNA in Ehrlich ascites cell nuclei is shown to be dependent upon the presence of both ATP and ADP. ATP can be replaced only by dATP. An ATP regenerating system is less effective than ATP alone or ATP in combination with ADP. ATP does not stimulate DNA synthesis primarily by maintenance of deoxyribonucleotide triphosphate levels. When the inhibition of DNA synthesis by high ATP levels is taken into account, the ATP analogs adenosine 5'-(alpha,beta-methylene)triphosphate, adenosine 5'-(beta, gamma-methylene)-triphosphate, and adenosine 5'-(beta, gamma-imino)triphosphate can neither substitute for ATP nor inhibit the ATP stimulation of DNA synthesis. Adenosine 5'-(3-thio)triphosphate, however, is a competitive inhibitor of DNA synthesis.

摘要

艾氏腹水细胞核中DNA的最佳合成显示依赖于ATP和ADP的同时存在。ATP只能被dATP替代。ATP再生系统比单独的ATP或ATP与ADP联合使用的效果要差。ATP主要不是通过维持脱氧核糖核苷三磷酸水平来刺激DNA合成。当考虑到高ATP水平对DNA合成的抑制作用时，ATP类似物5'-（α，β-亚甲基）三磷酸腺苷、5'-（β，γ-亚甲基）三磷酸腺苷和5'-（β，γ-亚氨基）三磷酸腺苷既不能替代ATP，也不能抑制ATP对DNA合成的刺激作用。然而，5'-（3-硫代）三磷酸腺苷是DNA合成的竞争性抑制剂。