Coassolo P, Aubert C, Cano J P
J Chromatogr. 1985 Mar 22;338(2):347-55. doi: 10.1016/0378-4347(85)80105-5.
A capillary gas-liquid chromatographic method for the determination of 3-methylclonazepam in plasma was developed. This method involved a single extraction by butyl acetate followed by analysis of the organic extract on a CP-Sil 5 glass capillary column with detection by electron capture. The detection limit was about 0.1 ng/ml, and the inter- and intra-assay precision did not exceed 8% for the concentration range 0.1-6.0 ng/ml. Specificity towards some of the possible metabolites in human plasma was demonstrated. This method was used for the measurement of the pharmacokinetic parameters of 3-methylclonazepam in healthy volunteers after a single intravenous administration of 1 mg, and oral administrations of 1 and 4 mg.
建立了一种测定血浆中3-甲基氯硝西泮的毛细管气-液色谱法。该方法包括用乙酸丁酯单次萃取，然后将有机萃取物在CP-Sil 5玻璃毛细管柱上进行分析，并通过电子捕获检测。检测限约为0.1 ng/ml，在0.1-6.0 ng/ml浓度范围内，批间和批内精密度均不超过8%。证明了该方法对人血浆中一些可能代谢物的特异性。该方法用于单次静脉注射1 mg、口服1 mg和4 mg后健康志愿者体内3-甲基氯硝西泮药代动力学参数的测定。
