Arianmanesh Mitra, Hassani Fatemeh, Karimian Leila, Yazdi Poopak Eftekhari, Movaghar Bahar, Ebrahimi Bita, Fakhri Mostafa, Valojerdi Mojtaba Rezazadeh
Department of Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Embryology, Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
JBRA Assist Reprod. 2025 Feb 21;29(2):289-97. doi: 10.5935/1518-0557.20240106.
Morphologic assessment of an embryo is a valuable indicator for determining embryo health; however, it does not provide information on the chromosomal status of an embryo. Therefore, this study aimed to investigate the levels of Apolipoprotein A1 secreted by day-5 embryos in the spent media of euploid and aneuploid human embryos.
This study utilized 131 spent culture media samples from 22 infertile couples who were referred to the fertility clinic of Royan Institute. Following ovulation induction, retrieved oocytes were fertilized by intracytoplasmic sperm injection. For pre-implantation genetic diagnosis, embryos were frozen and thawed on days 2 to 3 and a single blastomere was isolated from each embryo for the assessment of chromosomal abnormalities by fluorescence in situ hybridization. Five days after fertilization, the levels of Apolipoprotein A1 were determined in the spent media of normal embryos, aneuploid embryos (with chromosome abnormalities), and the control group (medium without any embryos) using enzyme-linked immunosorbent assay.
The Apolipoprotein A1 levels in the secretome of euploid cleavage-arrested embryos were significantly lower than those in the control group (p<0.04). However, Apolipoprotein A1 levels increased significantly in groups of euploid blastocysts, aneuploid cleavage-arrested embryos, aneuploid morulae, and aneuploid blastocysts compared to the control group (p<0.04). Furthermore, the Apolipoprotein A1 levels in the spent media of euploid early blastocysts were significantly higher compared to euploid hatching blastocysts and aneuploid blastocysts in the early, mid, and late stages (p<0.03).
This study highlights the significant potential of Apolipoprotein A1 as a developmental bi-omarker to distinguish between euploid and aneuploid embryos.
胚胎形态学评估是确定胚胎健康状况的重要指标；然而，它并不能提供胚胎染色体状态的信息。因此，本研究旨在调查整倍体和非整倍体人类胚胎的废弃培养液中第5天胚胎分泌的载脂蛋白A1水平。
本研究使用了来自22对不孕夫妇的131份废弃培养液样本，这些夫妇被转诊至罗扬研究所生育诊所。在诱导排卵后，通过胞浆内单精子注射使回收的卵母细胞受精。对于植入前基因诊断，胚胎在第2至3天冷冻和解冻，从每个胚胎中分离出一个卵裂球，通过荧光原位杂交评估染色体异常情况。受精后第5天，使用酶联免疫吸附测定法测定正常胚胎、非整倍体胚胎（有染色体异常）和对照组（无胚胎的培养液）的废弃培养液中载脂蛋白A1的水平。
整倍体卵裂期阻滞胚胎的分泌蛋白组中载脂蛋白A1水平显著低于对照组（p<0.04）。然而，与对照组相比，整倍体囊胚、非整倍体卵裂期阻滞胚胎、非整倍体桑葚胚和非整倍体囊胚组中的载脂蛋白A1水平显著升高（p<0.04）。此外，与整倍体孵化囊胚以及早期、中期和晚期的非整倍体囊胚相比，整倍体早期囊胚的废弃培养液中载脂蛋白A1水平显著更高（p<0.03）。
本研究突出了载脂蛋白A1作为区分整倍体和非整倍体胚胎的发育生物标志物的巨大潜力。
