Ercolani F, Morera E, Panichi G, Occhionero M, Luzzi I, Gianfrilli Mastrantonio P
Ann Ist Super Sanita. 1979;15(1):77-84.
A crucial factor affecting the ultimate success of anaerobic cultures is a proper specimen collection, with care to avoid inclusion of normal flora often present on human mucosa. In general, material for anaerobic cultures is best obtained using a needle and syringe, from which the air must be expelled. Then the specimens should be placed immediately into an anaerobic transport broth and then in suitable culture pre-reduced media.
影响厌氧培养最终成功的一个关键因素是正确采集标本，要注意避免混入人体黏膜上常见的正常菌群。一般来说，厌氧培养的材料最好用针筒采集，采集后必须排出空气。然后应立即将标本放入厌氧运送肉汤中，再置于合适的预还原培养基中培养。
