Dowell V R
Am J Med Technol. 1975 Nov;41(11):402-10.
Major points to be emphasized include: 1) Anaerobes are very numerous in nature, but only a relatively small number of species are commonly associated with disease in man; 2) The majority of anaerobic bacterial infections involve endogenous rather than exogenous microorganisms, and the patients are usually compromised in some manner prior to infection; and 3) Success in isolating anaerobic bacteria requires careful performance and control of each step in the isolation procedure. These steps include selection and collection of clinical specimens, microscopic examinations, selection and use of primary isolation media, use of anaerobic systems, incubation of cultures and subculture of colonies to obtain pure culture isolates.
1）自然界中厌氧菌数量众多，但通常只有相对少数的种类与人类疾病相关；2）大多数厌氧细菌感染涉及内源性而非外源性微生物，并且患者在感染前通常在某种程度上存在身体机能受损的情况；3）成功分离厌氧菌需要在分离过程中的每个步骤都认真操作和控制。这些步骤包括临床标本的选择和采集、显微镜检查、初代分离培养基的选择和使用、厌氧培养系统的使用、培养物的孵育以及菌落的传代培养以获得纯培养分离物。
