Cecchini G, Tessitore L, Baccino F M
Boll Soc Ital Biol Sper. 1979 Jul 15;55(12):1130-5.
Methodological difficulties limit studies on cell protein catabolism both in intact animals and in vitro. We have studied the rate of protein degradation by measuring in vitro the release of acid-soluble radioactivity from rat liver slices and tested some factors that control the process. We found a rate of protein degradation of 6.5, or 2% per hr after 1 or 15 hrs of labelling in vivo during the first 90 min. These results indicate that a correlation exists between the rate of production of acid-soluble radioactivity by liver slices and the fast-or slow-turnover rate of the liver proteins. Cyanide and fluoride greatly inhibit the production of acid-soluble radioactivity from both slow- and fast-turnover proteins. Glucagon increases this production while insulin shows an opposite effect. Our preliminary investigations show that liver slices are a suitable surviving medium to study protein catabolism and its modifications under physiological and pathological stimuli.
方法学上的困难限制了对完整动物体内和体外细胞蛋白质分解代谢的研究。我们通过体外测量大鼠肝切片中酸溶性放射性物质的释放来研究蛋白质降解速率，并测试了一些控制该过程的因素。我们发现在体内标记1小时或15小时后的最初90分钟内，蛋白质降解速率为6.5，即每小时2%。这些结果表明，肝切片产生酸溶性放射性物质的速率与肝脏蛋白质的快速或慢速周转速率之间存在相关性。氰化物和氟化物极大地抑制了慢速和快速周转蛋白质产生酸溶性放射性物质。胰高血糖素增加了这种产生，而胰岛素则表现出相反的作用。我们的初步研究表明，肝切片是研究蛋白质分解代谢及其在生理和病理刺激下修饰的合适存活介质。
