Lyaruu D M, Bronckers A L, Burger E H, Wöltgens J H
J Histochem Cytochem. 1985 Jun;33(6):595-603. doi: 10.1177/33.6.3998459.
Potassium pyroantimonate-osmium tetroxide cytochemistry has been used to study the distribution of ionic calcium in hamster tooth germs during cell differentiation and during early dentinogenesis and amelogenesis. Before the onset of mineralization, pyroantimonate (PA) reaction product was found in the nucleus of differentiating preameloblasts and preodontoblasts. In the predentin, it was preferentially located along striated collagen fibrils, lying perpendicular to the basal lamina. At the onset of mineralization, a pronounced increase of PA reaction product was evident in the predentin and on the plasma membrane and in mitochondria of both preodontoblasts and preameloblasts opposite the mineralizing mantle dentin. During early enamel mineralization, PA reaction product was present in the "growing" crystal ends, while in the secretory ameloblasts, most of the PA reaction product was localized on the cytoplasmic side of the apical plasma membranes and in mitochondria. When Tomes' processes developed, PA reaction product, both cytoplasmic and membrane bound, was low or absent deep in the processes, but gradually increased toward the apical terminal web. A corresponding gradient of PA reaction product was observed on the opposing enamel crystallites. From this study we conclude that both preodontoblasts and preameloblasts seem to be involved in calcium acquisition necessary for the early stages of mantle dentin mineralization. Tomes' processes seem to regulate the entry of calcium into the enamel mineralization front.
焦锑酸钾-四氧化锇细胞化学已被用于研究仓鼠牙胚在细胞分化、早期牙本质形成和釉质形成过程中离子钙的分布。在矿化开始之前，在分化中的成釉细胞前体细胞和成牙本质细胞前体细胞的细胞核中发现了焦锑酸盐（PA）反应产物。在前期牙本质中，它优先沿着与基膜垂直的横纹胶原纤维分布。在矿化开始时，在前期牙本质以及与矿化罩牙本质相对的成牙本质细胞前体细胞和成釉细胞前体细胞的质膜和线粒体上，PA反应产物明显增加。在早期釉质矿化过程中，PA反应产物存在于“生长”的晶体末端，而在分泌期成釉细胞中，大部分PA反应产物位于顶端质膜的细胞质侧和线粒体中。当托姆斯突形成时，细胞质和膜结合的PA反应产物在突的深部含量低或不存在，但向顶端终末网逐渐增加。在相对的釉质微晶上观察到PA反应产物的相应梯度。从这项研究中我们得出结论，成牙本质细胞前体细胞和成釉细胞前体细胞似乎都参与了罩牙本质矿化早期阶段所需的钙摄取。托姆斯突似乎调节钙进入釉质矿化前沿。
