Fujioka-Kobayashi Masako, Urbanova Veronika, Lang Niklaus P, Katagiri Hiroki, Saulacic Nikola
Department of Cranio-Maxillofacial Surgery, Inselspital, Bern University Hospital, Bern, Switzerland.
Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
BMC Oral Health. 2025 Feb 21;25(1):275. doi: 10.1186/s12903-025-05644-9.
To study the effect on biomaterial degradation and bone formation of different ratios between α-tricalcium phosphate (α-TCP) and deproteinized bovine bone mineral (DBBM) using various gelatins as a carrier.
Thirty-six critical-sized calvarial bone defects were randomly treated in 18 animals. Four biomaterials with different compositional relations of DBBM to α-TCP and granules to carrier were investigated: (1) 40 ± 10% DBBM/40 ± 10% α-TCP with 20 ± 10% gelatin type 1 in ratio 4:1 (B1/G1), (2) 20 ± 10% DBBM/60 ± 10% α-TCP with 20 ± 10% gelatin type 1 in ratio 4:1 (B2/G1), (3) 20 ± 10% DBBM/60 ± 10% α-TCP with 15 ± 10%/5 ± 5% gelatin type 2/ glycerine (B2/G2) and 4), 10 ± 10% DBBM/60 ± 10% α-TCP with 20 ± 10%/10 ± 10% gelatin type 2/ glycerine (B3/G2). As a positive control 50 ± 10% DBBM/50 ± 10% α-TCP without gelatin (PC, B1/G0) and as a negative control (NC) empty defects were chosen. All defects were covered with a collagen membrane. The samples were harvested 4 weeks post-surgically and examined by micro-CT and histomorphometric analysis.
New bone formation was evident in all defects. The mineralized tissue volume was significantly higher in the four tested biomaterials than in the NC group, but lower compared to the PC group. Histomorphometry showed similar levels of bone formation in all groups, whereas only the PC group reached a significantly higher total augmentation area than that of the NC. The PC group showed significantly higher mineralized tissue density and residual material area compared to the B3/G2 group, and more residual DBBM than the four tested biomaterials.
New bone formation was not significantly affected either by different DBBM:α-TCP compositional ratios nor the presence of various gelatin carriers.
Similar levels of osteoconductivity indicates the presumptive use of combined products in alveolar ridge augmentation to support bone formation. Gelatin with or without glycerine may be considered for its use as a carrier to the biomaterials frequently applied in peri-implant surgery.
研究以不同明胶为载体时，α - 磷酸三钙（α - TCP）与脱蛋白牛骨矿物质（DBBM）不同比例对生物材料降解及骨形成的影响。
对18只动物的36个临界尺寸的颅骨骨缺损进行随机处理。研究了四种具有不同DBBM与α - TCP组成关系以及颗粒与载体比例的生物材料：（1）40±10% DBBM/40±10% α - TCP与20±10% 1型明胶，比例为4:1（B1/G1）；（2）20±10% DBBM/60±10% α - TCP与20±10% 1型明胶，比例为4:1（B2/G1）；（3）20±10% DBBM/60±10% α - TCP与15±10%/5±5% 2型明胶/甘油（B2/G2）；（4）10±10% DBBM/60±10% α - TCP与20±10%/10±10% 2型明胶/甘油（B3/G2）。作为阳性对照，选用50±10% DBBM/50±10% α - TCP且无明胶（PC，B1/G0），作为阴性对照（NC）则选择空白缺损。所有缺损均覆盖胶原膜。术后4周采集样本，通过显微CT和组织形态计量学分析进行检测。
所有缺损处均有明显的新骨形成。四种测试生物材料中的矿化组织体积显著高于NC组，但低于PC组。组织形态计量学显示所有组的骨形成水平相似，而只有PC组的总增大面积显著高于NC组。与B3/G2组相比，PC组的矿化组织密度和残余材料面积显著更高，且残余DBBM比四种测试生物材料更多。
不同的DBBM:α - TCP组成比例以及各种明胶载体的存在对新骨形成均无显著影响。
相似的骨传导水平表明联合产品在牙槽嵴增高术中可能用于支持骨形成。含或不含甘油的明胶可被考虑用作种植体周围手术中常用生物材料的载体。
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