Khan Sadia, Ouidir Marion, Lemaitre Nicolas, Jovanovic Nicolas, Bayat Sam, Lyon-Caen Sarah, Hoffmann Pascale, Desseux Morgane, Thomsen Cathrine, Couturier-Tarrade A, Småstuen Haug Line, Valmary-Degano Séverine, Siroux Valérie, Slama Rémy, Alfaidy Nadia, Philippat Claire
University Grenoble Alpes, Inserm U-1209, CNRS-UMR-5309, Environmental Epidemiology Applied to Reproduction and Respiratory Health Team, Institute for Advanced Biosciences, 38000, Grenoble, France.
Commissariat à l'Energie Atomique et aux Energies Alternatives, CNRS, Inserm U1292, Biosciences and Biotechnology Institute of Grenoble, Université Grenoble Alpes, Grenoble, France.
Environ Int. 2025 Mar;197:109308. doi: 10.1016/j.envint.2025.109308. Epub 2025 Jan 30.
Animal studies have linked prenatal poly- and perfluoroalkyl substances (PFAS) exposures with impaired placental structure and function. In humans, only few studies have investigated such associations.
We studied whether PFAS, individually and as a mixture, affected placental function.
In 367 pregnant women, we quantified 13 PFAS in serum collected at 19.3 gestational weeks (median). Placental weight was recorded at delivery. Histological examination of placental tissues allowed estimation of vascular perfusion (percentage of villi with syncytial knots, capillary density, intervillous space) and placental aging (fibrin deposition, calcification). Associations between PFAS and each of these parameters were assessed using adjusted linear, logistic regressions and mixture modeling through cluster analysis and Bayesian kernel machine regression (BKMR).
PFHxPA quantification (yes versus no) was associated with an increase in the percentages of villi with syncytial knots (β = 6.0% [95% CI: 1.1; 11]) and reduced intervillous spaces (β = 4.7% [95% CI: 0.1; 9.3]). A similar pattern was observed with PFHpA. Isolated associations were observed between PFTrDA and percentages of villi with syncytial knots (β = 8.6% [95% CI: 2.2; 15]) and 6:2diPAP and capillary density (β = -17% [95% CI: -30; -4.6]). Cluster analysis suggested that women in the moderate-to-higher PFAS exposure group had on average lower placental weight (β= -30 g [95% CI: -56; -4.3]), compared to those in the lower exposure group.
Pregnancy PFAS levels were associated with placental parameters of fetal-maternal exchange, highlighting their broad physiological impacts.
动物研究表明，产前多氟和全氟烷基物质（PFAS）暴露与胎盘结构和功能受损有关。在人类中，只有少数研究调查了此类关联。
我们研究了PFAS单独及作为混合物时是否会影响胎盘功能。
在367名孕妇中，我们对妊娠19.3周（中位数）时采集的血清中的13种PFAS进行了定量。分娩时记录胎盘重量。对胎盘组织进行组织学检查，以评估血管灌注（有合体结节的绒毛百分比、毛细血管密度、绒毛间隙）和胎盘老化（纤维蛋白沉积、钙化）。使用调整后的线性回归、逻辑回归以及通过聚类分析和贝叶斯核机器回归（BKMR）的混合物建模，评估PFAS与这些参数之间的关联。
PFHxPA定量（有与无）与有合体结节的绒毛百分比增加（β = 6.0% [95%置信区间：1.1；11]）和绒毛间隙减小（β = 4.7% [95%置信区间：0.1；9.3]）有关。PFHpA也观察到类似模式。在PFTrDA与有合体结节的绒毛百分比（β = 8.6% [95%置信区间：2.2；15]）以及6:2diPAP与毛细血管密度（β = -17% [95%置信区间：-30；-4.6]）之间观察到单独的关联。聚类分析表明，与低暴露组相比，中高PFAS暴露组的女性平均胎盘重量较低（β = -30 g [95%置信区间：-56；-4.3]）。
孕期PFAS水平与母婴交换的胎盘参数有关，突出了它们广泛的生理影响。
