Peng Shiqiang, Lin Lianzhu, Zhao Mouming
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, China.
Food Res Int. 2025 Mar;204:115825. doi: 10.1016/j.foodres.2025.115825. Epub 2025 Feb 6.
Drinking tea is beneficial to reduce the incidence of obesity. Unlike Fuzhuan brick tea (DT, a typical dark tea) widely reported to have good glycolipid-lowering activity (GLA), there is little information on GLA of Dancong summer tea (OT, a typical oolong tea). A comparative study on GLA and chemical compositions of OT and DT was systematically conducted. The results showed that OT had advantages in high content of polyphenols, leading to its better GLA including glycolipid digestion enzyme inhibitory activity and cholesterol micellar solubility inhibition activity than DT's. A total of 21 compounds mainly including catechins, flavonoids, phenolic acids and phenylpropanoids were screened as the key differentially active micromolecules (KDAM) by UPLC-QTOF-MS/MS-based untargeted metabolomic analysis combined with multivariate analysis, which resulted in the difference of GLA between OT and DT. Moreover, the high affinity capacity and interaction mode between KDAM with α-glucosidase/α-amylase/pancreatic lipase/cholesterol esterase were validated by molecular docking. Although the accumulation of polyphenols makes OT has a poor flavor as a beverage, our research demonstrated that OT with high content of polyphenols could be used in functional foods to facilitate the prevention of obesity.
喝茶有助于降低肥胖发生率。与广泛报道具有良好降血脂活性（GLA）的茯砖茶（DT，一种典型的黑茶）不同，关于单枞夏茶（OT，一种典型的乌龙茶）的GLA的信息很少。对OT和DT的GLA及化学成分进行了系统的比较研究。结果表明，OT在多酚含量高方面具有优势，导致其GLA优于DT，包括糖脂消化酶抑制活性和胆固醇胶束溶解性抑制活性。通过基于超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）的非靶向代谢组学分析结合多变量分析，共筛选出21种主要包括儿茶素、黄酮类、酚酸和苯丙素类的化合物作为关键差异活性小分子（KDAM），这导致了OT和DT之间GLA的差异。此外，通过分子对接验证了KDAM与α-葡萄糖苷酶/α-淀粉酶/胰脂肪酶/胆固醇酯酶之间的高亲和力和相互作用模式。尽管多酚的积累使OT作为饮料口感不佳，但我们的研究表明，多酚含量高的OT可用于功能性食品中，以促进肥胖的预防。
