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大鼠脑成熟过程中磷脂甲基酶活性、[3H]S-腺苷-L-高半胱氨酸结合以及S-腺苷-L-甲硫氨酸和S-腺苷-L-高半胱氨酸水平

Phospholipid methylase activity, [3H]S-adenosyl-L-homocysteine binding, and S-adenosyl-L-methionine and S-adenosyl-L-homocysteine levels in rat brain during maturation.

作者信息

Gharib A, Rey C, Fonlupt P, Sarda N, Pacheco H

出版信息

J Neurochem. 1985 Jul;45(1):32-6. doi: 10.1111/j.1471-4159.1985.tb05470.x.

DOI:10.1111/j.1471-4159.1985.tb05470.x

PMID:3998730

Abstract

The changes in activity of phospholipid methyltransferase I and [3H]S-adenosyl-L-homocysteine ([3H]SAH) binding were determined in cortical membrane preparations from newborn rats and rats 1, 2, and 8 months old. The activity of phospholipid methyltransferase I and the [3H]SAH binding were significantly greater (respectively, +30 and +40%) in newborn rats than in 1-, 2-, and 8-month-old rats. The methylated products at days 1 and 30 were identical. These changes in methyltransferase activity may be correlated with variations in concentration of S-adenosyl-L-methionine (SAM) and SAH. The endogenous SAM level was higher and the SAH level was lower in newborn compared with adult rats. These data suggested that the processes of methylation were favored in newborn rats. The modifications observed after treatment with L-homocysteine reinforced this hypothesis.

摘要

测定了新生大鼠以及1、2和8月龄大鼠的皮质膜制剂中磷脂甲基转移酶I的活性变化和[3H]S-腺苷-L-高半胱氨酸（[3H]SAH）结合情况。新生大鼠中磷脂甲基转移酶I的活性和[3H]SAH结合显著高于1、2和8月龄大鼠（分别高出30%和40%）。第1天和第30天的甲基化产物相同。甲基转移酶活性的这些变化可能与S-腺苷-L-甲硫氨酸（SAM）和SAH浓度的变化相关。与成年大鼠相比，新生大鼠内源性SAM水平较高而SAH水平较低。这些数据表明新生大鼠中甲基化过程更有利。用L-高半胱氨酸处理后观察到的变化强化了这一假设。