Sarda N, Reynaud D, Gharib A
Department of Biological Chemistry, INSERM U205, Villeurbanne, France.
Dev Pharmacol Ther. 1989;13(2-4):104-12. doi: 10.1159/000457591.
The changes in activity of N-methyltransferase 1 and [3H]-S-adenosylhomocysteine binding were determined in cortical membranes preparations from newborn and 1-, 2- and 10-month-old rats. These parameters were significantly higher: +130 and 200%, respectively, in 1-day compared to 2-month-old rats (adult). With maturation, S-adenosylmethionine levels decreased (-24%) whereas adenosine levels increased gradually from 0.17 to 6.10 nmol/mg protein in forebrain or whole brain between 1-day and 10-month-old rats. The ontogenesis of adenosine receptors was studied. The A2 receptors were not detectable at 1 day. For A1 receptors, Kd and Bmax increased with age while for A2 receptors Kd and Bmax were similar between 9 days and 2 months of age.
测定了新生大鼠以及1、2和10月龄大鼠的皮质膜制剂中N-甲基转移酶1的活性变化和[3H]-S-腺苷同型半胱氨酸结合情况。与2月龄大鼠（成年）相比，这些参数在1日龄大鼠中显著更高，分别提高了130%和200%。随着成熟，S-腺苷甲硫氨酸水平下降（-24%），而在1日龄至10月龄大鼠的前脑或全脑中，腺苷水平从0.17逐渐增加至6.10 nmol/mg蛋白质。研究了腺苷受体的个体发生。在1日龄时未检测到A2受体。对于A1受体，Kd和Bmax随年龄增加，而对于A2受体，在9日龄至2月龄之间Kd和Bmax相似。
