Hu Liming, Wang Peng, Wan Lingfei, Yan Xinlong, Mo Shanyan
College of Chemistry and Life Science, Beijing Key Laboratory of Environmental and Viral Oncology, Beijing University of Technology, Beijing 100124, China.
College of Chemistry and Life Science, Beijing Key Laboratory of Environmental and Viral Oncology, Beijing University of Technology, Beijing 100124, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jun 5;334:125908. doi: 10.1016/j.saa.2025.125908. Epub 2025 Feb 16.
Fluorescence imaging holds great potential as a powerful diagnostic tool for tumor cell visualization. However, a significant challenge in fluorescence imaging is the high background signal, which obscures the tumor-specific signal and reduced the signal-to-noise ratio (SNR) of imaging, thereby reducing the accuracy of tumor detection and delineation. In this research, we designed and synthesized an amphiphilic disulfide dicyanine ss-diCy7, which can self-assemble into nanoparticles with uniform dispersion in aqueous environments. The fluorescence intensity of these nanoparticles is significantly reduced by 96% due to aggregation-induced quenching arising from π-π stacking. The nanoparticles exhibit a highly specific response to glutathione (GSH) in vitro, resulting in a substantial enhancement of fluorescence intensity by a 24-fold. The enhancement was also achieved in cell and mouse imaging experiments. In addition, in the mouse tumor model, ss-diCy7 nanoparticles demonstrated superior performance compared to traditional mono-cyanine dyes, offering a reduced background signal and prolonged fluorescence duration. This work is anticipated to contribute to the high-resolution tumor imaging.
荧光成像作为一种用于肿瘤细胞可视化的强大诊断工具具有巨大潜力。然而，荧光成像中的一个重大挑战是高背景信号，它会掩盖肿瘤特异性信号并降低成像的信噪比（SNR），从而降低肿瘤检测和描绘的准确性。在本研究中，我们设计并合成了一种两亲性二硫代双菁ss-diCy7，它可以在水性环境中自组装成具有均匀分散性的纳米颗粒。由于π-π堆积引起的聚集诱导猝灭，这些纳米颗粒的荧光强度显著降低了96%。纳米颗粒在体外对谷胱甘肽（GSH）表现出高度特异性反应，导致荧光强度大幅增强24倍。在细胞和小鼠成像实验中也实现了这种增强。此外，在小鼠肿瘤模型中，ss-diCy7纳米颗粒与传统单菁染料相比表现出卓越性能，提供了更低的背景信号和更长的荧光持续时间。这项工作有望为高分辨率肿瘤成像做出贡献。
