Honda C N
J Neurophysiol. 1985 Apr;53(4):1059-78. doi: 10.1152/jn.1985.53.4.1059.
One hundred and sixty extracellularly and intracellularly recorded unitary discharges from the sacral or caudal spinal segments of 30 anemically decerebrated cats were studied to examine the effects of somatic and visceral afferent stimulation on neurons near the central canal (CC). The recorded unitary activity was histologically verified (by dye marks or horseradish peroxidase, HRP) as having come from the gray matter surrounding the CC that approximates Rexed's lamina X. In the absence of intentional stimulation or apparent injury by the recording electrode, 62% of the units exhibited ongoing discharges. Each unit was tested for responses to the stimulation of somatic (cutaneous and subcutaneous) and visceral (bladder and colon) structures. Seventy-six (48%) of the units responded exclusively to the stimulation of somatic receptive fields, and 10 (6%) of the units were selectively responsive to stimulation of the pelvic viscera. The activity of the remaining 74 (46%) was influenced by activity in both somatic and visceral afferent fibers. Eighteen of the 160 neurons were intracellularly marked with HRP. Based on perikaryal size and dendritic extent, it was possible to divide these cells into two partially overlapping groups. One group consisted of seven neurons with small to medium-sized perikarya, dendritic arbors largely restricted to the gray matter surrounding the CC, and small, singular somatic receptive fields. The second group comprised 11 cells with medium to large-sized soma and dendrites extending out of lamina X. These larger neurons usually possessed multiple, widely distributed somatic receptive fields. The principal finding of the present study is that in the sacral spinal cord many cells near the CC receive primary afferent inputs converging from a wide range of receptor types in somatic and visceral structures. Such neurons are capable of integrating afferent information from somatic structures on both sides of the body with information originating in pelvic viscera and midline regions such as the genitals.
对30只贫血去大脑猫的骶段或尾段脊髓节段进行了160次细胞外和细胞内记录的单位放电研究，以检查躯体和内脏传入刺激对中央管（CC）附近神经元的影响。记录的单位活动经组织学验证（通过染料标记或辣根过氧化物酶，HRP），确定来自CC周围灰质，该灰质近似于Rexed板层X。在没有故意刺激或记录电极明显损伤的情况下，62%的单位表现出持续放电。对每个单位进行了对躯体（皮肤和皮下）和内脏（膀胱和结肠）结构刺激的反应测试。76个（48%）单位仅对躯体感受野的刺激有反应，10个（6%）单位对盆腔内脏刺激有选择性反应。其余74个（46%）的活动受到躯体和内脏传入纤维活动的影响。160个神经元中有18个用HRP进行了细胞内标记。根据胞体大小和树突范围，可将这些细胞分为两个部分重叠的组。一组由7个神经元组成，其胞体小至中等大小，树突主要局限于CC周围的灰质，且有小的、单一的躯体感受野。第二组由11个细胞组成，其胞体中等至大，树突延伸出板层X。这些较大的神经元通常具有多个广泛分布的躯体感受野。本研究的主要发现是，在骶段脊髓中，CC附近的许多细胞接收来自躯体和内脏结构中广泛受体类型的初级传入输入汇聚。这样的神经元能够将来自身体两侧躯体结构的传入信息与来自盆腔内脏和中线区域（如生殖器）的信息整合起来。
