Sui Mingming, Zhang Fuman, Tian Tian, Yan Yanqiu, Geng Le, Li Hui, Bai Yu'e
College of Forestry, Inner Mongolia Agricultural University, Hohhot 010000, Inner Mongolia, China.
Sheng Wu Gong Cheng Xue Bao. 2025 Feb 25;41(2):825-844. doi: 10.13345/j.cjb.240699.
, known for its remarkable tolerance to cold, drought, and salinity, is a key species for ecological restoration and urban greening in the "Three Norths" region of China. MYB transcription factors are involved in plant responses to abiotic stress and synthesis of secondary metabolites. However, studies are limited regarding the MYB transcription factors in . and their roles in salt stress tolerance. In this study, 196 MYBs were identified based on the genome of and the transcriptome of . . Phylogenetic analysis classified the MYB transcription factors into seven subclasses. The R2R3-MYB subclass contained the maximum number of genes (84.77%), while the R-R and R1R2R3 subclasses each represented the smallest proportion, at about 0.51%. The MYB transcription factors within the same subclass were highly conserved, exhibiting similar motifs and gene structures. Experiments with varying salt stress gradients revealed that . could tolerate the salt concentration up to 1 000 mmol/L. From the transcriptome data of . exposed to salt stress (1 000 mmol/L) for 0, 3, 6, 12, and 24 h, a total of 34 differentially expressed MYBs were identified, which suggested that these MYBs played a key role in regulating the response to salt stress. The proteins encoded by these differentially expressed genes varied in length from 89 aa to 731 aa, with molecular weights ranging from 10.19 kDa to 79.73 kDa, isoelectric points between 4.80 and 9.91, and instability coefficients from 41.20 to 70.99. Subcellular localization analysis indicated that most proteins were localized in the nucleus, while three were found in the chloroplasts. Twelve MYBs were selected for quantitative real-time PCR (qRT-PCR), which showed that their expression patterns were consistent with the RNA-seq data. This study provides valuable data for further investigation into the functions and mechanisms of MYB family members in response to salt stress in . .
以其对寒冷、干旱和盐度的显著耐受性而闻名，是中国“三北”地区生态恢复和城市绿化的关键物种。MYB转录因子参与植物对非生物胁迫的响应和次生代谢产物的合成。然而，关于该物种中MYB转录因子及其在耐盐胁迫中的作用的研究有限。在本研究中，基于该物种的基因组和转录组鉴定出196个MYB。系统发育分析将MYB转录因子分为七个亚类。R2R3-MYB亚类包含的基因数量最多（84.77%），而R-R和R1R2R3亚类各自占比最小，约为0.51%。同一亚类中的MYB转录因子高度保守，表现出相似的基序和基因结构。不同盐胁迫梯度的实验表明，该物种能够耐受高达1000 mmol/L的盐浓度。从该物种在0、3小时、6小时、12小时和24小时暴露于盐胁迫（1000 mmol/L）的转录组数据中，共鉴定出34个差异表达的MYB，这表明这些MYB在调节对盐胁迫的响应中起关键作用。这些差异表达基因编码的蛋白质长度从89个氨基酸到731个氨基酸不等，分子量范围为10.19 kDa至79.73 kDa，等电点在4.80至9.91之间，不稳定系数在41.20至70.99之间。亚细胞定位分析表明，大多数蛋白质定位于细胞核，而有三个定位于叶绿体。选择了12个MYB进行定量实时PCR（qRT-PCR），结果表明它们的表达模式与RNA-seq数据一致。本研究为进一步研究该物种中MYB家族成员响应盐胁迫的功能和机制提供了有价值的数据。
