Butsch Tyler J, Johnson Alyssa E, Bohnert K Adam
Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States.
MicroPubl Biol. 2025 Feb 7;2025. doi: 10.17912/micropub.biology.001509. eCollection 2025.
Lysosome activity regulates germline development in multiple species. In the testis, lysosomes activate as germ cells exit mitosis and enter meiosis. Notably, reduced activity of germ-cell lysosomes, which is seen during aging, leads to fewer viable sperm. Here, we investigated the activity of Mitf/TFEB, a master regulator of lysosome biogenesis, during spermatogenesis. We discovered that Mitf activity was upregulated in meiotic-stage spermatocytes, consistent with the lysosome-activation pattern. However, Mitf activity in spermatocytes declined in older males, concurrent with reduced expression of a Mitf-targeted V-ATPase component. These findings provide insight into the regulation of upstream lysosome controls during spermatogenesis.
溶酶体活性调节多种物种的生殖细胞发育。在睾丸中，随着生殖细胞退出有丝分裂并进入减数分裂，溶酶体被激活。值得注意的是，生殖细胞溶酶体活性降低（在衰老过程中可见）会导致有活力的精子数量减少。在这里，我们研究了溶酶体生物发生的主要调节因子Mitf/TFEB在精子发生过程中的活性。我们发现，Mitf活性在减数分裂期的精母细胞中上调，这与溶酶体激活模式一致。然而，老年雄性动物精母细胞中的Mitf活性下降，同时Mitf靶向的V-ATP酶成分的表达也降低。这些发现为精子发生过程中上游溶酶体控制的调节提供了见解。
