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微流控芯片设计及其在红细胞E抗原分型中的应用。

Microfluidic chip designs and their application for E antigen typing on red blood cells.

作者信息

Maraming Pornsuda, Shean Aye Nang Noon, Panyakakaew Pattarawarin, Tippayawat Patcharaporn, Daduang Sakda, Choowongkomon Kiattawee, Jearanaikoon Nichada, Srisom Kriettisak, Phatthanakun Rungrueang, Daduang Jureerut

机构信息

Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University Khon Kaen 40002 Thailand

Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University Khon Kaen 40002 Thailand

出版信息

RSC Adv. 2025 Feb 24;15(8):6077-6088. doi: 10.1039/d4ra08321k. eCollection 2025 Feb 19.

DOI:10.1039/d4ra08321k
PMID:39995458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11848836/
Abstract

The E antigen is one of the main Rh antigens on red blood cells (RBCs). The high ability of the E antigen to trigger an immune response along with the presence of anti-E, can cause serious issues such as hemolytic transfusion reactions and hemolytic disease in newborns. In this study, we developed a microfluidic biochip for Rh typing (E antigen). Three different micromixer types were compared for simulation, mixing tests, and optimal interactions of blood typing reagents and RBCs to provide an accurate agglutination reaction. Using separate syringe pumps, a blood sample and anti-E reagent were introduced into the microfluidic device through the respective inlet channels. Then, the fluids underwent thorough mixing within the micromixers before reaching reaction reservoirs where RBC agglutination was observed. The 8-shape micromixer design showed the most agglutination and indicated the best performance for E antigen detection and was able to differentiate blood clinical samples with E-positive and -negative RBCs. The microfluidic chip could also be applied for RBC antigen detection in blood bank procedures during blood typing and compatibility testing.

摘要

E抗原是红细胞（RBC）上主要的Rh抗原之一。E抗原引发免疫反应的能力很强，再加上抗E的存在，可能会导致严重问题，如溶血性输血反应和新生儿溶血病。在本研究中，我们开发了一种用于Rh血型鉴定（E抗原）的微流控生物芯片。比较了三种不同类型的微混合器，进行模拟、混合测试以及血型鉴定试剂与红细胞的最佳相互作用，以提供准确的凝集反应。使用单独的注射泵，将血液样本和抗E试剂通过各自的入口通道引入微流控装置。然后，流体在微混合器中充分混合，再到达反应容器，在那里观察红细胞凝集情况。8字形微混合器设计显示出最多的凝集现象，表明其在E抗原检测方面性能最佳，并且能够区分具有E阳性和E阴性红细胞的血液临床样本。该微流控芯片还可应用于血型鉴定和相容性检测过程中血库程序的红细胞抗原检测。

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