Kerdsuknirund Sasitorn, Kosinan Arreeya, Khunkaewla Panida, Kupittayanant Pakanit, Tongdee Pattama, Nimkuntod Porntip, Wray Susan, Kupittayanant Sajeera
School of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
Biochemistry-Electrochemistry Research Unit, School of Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
Curr Res Physiol. 2025 Jan 31;8:100139. doi: 10.1016/j.crphys.2025.100139. eCollection 2025.
This study investigates the effects of (L.) H. Rob. ethanolic extract (CCE) on gestational diabetes mellitus (GDM) in rats using biochemical, histological, and uterine contractility studies.
Diabetes was induced in pregnant rats using streptozotocin (60 mg/kg). CCE was administered orally at low (50 mg/kg BW) and high (500 mg/kg BW) doses from gestation day 7 to day 21. Maternal evaluations included body weight, gravid uterine weight, and biochemical assays for serum glucose, insulin, lipids, and liver enzymes. Fetal outcomes included fetal size. Histological analyses of maternal pancreatic and uterine tissues and uterine contractility studies using muscle strip experiments were also performed.
CCE and metformin (MET) significantly reduced elevated blood glucose levels and improved the Islets of Langerhans area compared to the GDM group ( < 0.05). Both treatments showed a trend toward increased insulin levels ( > 0.05) and significantly reduced lipids, AST, and ALP levels ( < 0.05). High-dose CCE and MET increased gravid uterine weight and fetal size ( < 0.05) while showing a trend toward reducing placental weight and index ( > 0.05). Histological analysis revealed increased fiber area and decreased interstitial space in uterine sections ( < 0.05). , CCE enhanced spontaneous and oxytocin-induced contractions ( < 0.05), while MET had no effect.
CCE reduces elevated glucose levels and exhibits hypolipidemic and hepatoprotective effects, improving maternal and fetal outcomes in GDM. Its uterine contractility effects suggest potential as a complementary therapy to MET for GDM management.
本研究通过生化、组织学和子宫收缩性研究，探讨罗布麻乙醇提取物（CCE）对大鼠妊娠期糖尿病（GDM）的影响。
用链脲佐菌素（60mg/kg）诱导妊娠大鼠患糖尿病。从妊娠第7天至第21天，以低剂量（50mg/kg体重）和高剂量（500mg/kg体重）口服CCE。母体评估包括体重、妊娠子宫重量，以及血清葡萄糖、胰岛素、脂质和肝酶的生化检测。胎儿结局包括胎儿大小。还进行了母体胰腺和子宫组织的组织学分析以及使用肌肉条实验进行子宫收缩性研究。
与GDM组相比，CCE和二甲双胍（MET）显著降低了升高的血糖水平，并改善了胰岛面积（P<0.05）。两种治疗均显示出胰岛素水平升高的趋势（P>0.05），并显著降低了脂质、AST和ALP水平（P<0.05）。高剂量CCE和MET增加了妊娠子宫重量和胎儿大小（P<0.05），同时显示出降低胎盘重量和胎盘指数的趋势（P>0.05）。组织学分析显示子宫切片中的纤维面积增加，间质空间减少（P<0.05）。此外，CCE增强了自发性和催产素诱导的收缩（P<0.05），而MET没有效果。
CCE降低升高的血糖水平，具有降血脂和保肝作用，改善了GDM的母体和胎儿结局。其子宫收缩性作用表明其有可能作为MET治疗GDM的辅助疗法。
