Truman Brennan, Ma Linda, Stewart Samuel, Kingsley Karl, Sullivan Victoria
Department of Advanced Education in Pediatric Dentistry, School of Dental Medicine, University of Nevada, 1700 West Charleston Blvd, Las Vegas, NV 89106, USA.
Department of Clinical Sciences, School of Dental Medicine, University of Nevada, 1700 West Charleston Blvd, Las Vegas, NV 89106, USA.
Methods Protoc. 2025 Feb 11;8(1):18. doi: 10.3390/mps8010018.
Many endodontic procedures within the pediatric population are performed with patients aged 12 years and older, using intracanal irrigants to complement mechanical debridement for the removal of debris and to disinfect the root canal system. The use of antimicrobial irrigants that limit damage to the dental pulp are the goals of endodontic biomaterials research. Using an existing biorepository of dental pulp stem cells (DPSCs), Endocyn was evaluated in varying concentrations in proliferation and viability assays, and compared with positive (sodium hypochlorite or bleach) and negative (phosphate-buffered saline) controls. The DPSC viability was reduced in the range of -8.3% to -15.8%, = 0.22 to = 0.042, while the growth inhibition varied between -29.7% and -63%, = 0.041 to = 0.022. However, the RNA analysis revealed that no significant changes in biomarker mRNA expression (Nestin, NANOG, Sox2, Oct4, CD73, CD90, and CD105) were observed. These data demonstrated that all of the concentrations of Endocyn inhibited the DPSC viability and growth, although only high concentrations were statistically significant. Moreover, the administration of Endocyn did not alter the DPSC biomarker expression, which are novel and important findings not previously observed or reported that may assist with the development of clinical decision protocols and methods for the treatment of vital pulp tissue.
许多针对儿童群体的牙髓治疗程序是针对12岁及以上的患者进行的，使用根管冲洗剂来辅助机械清创，以清除碎屑并消毒根管系统。使用对牙髓损伤较小的抗菌冲洗剂是牙髓生物材料研究的目标。利用现有的牙髓干细胞生物样本库，在增殖和活力测定中对不同浓度的Endocyn进行了评估，并与阳性对照（次氯酸钠或漂白剂）和阴性对照（磷酸盐缓冲盐水）进行了比较。牙髓干细胞的活力降低了-8.3%至-15.8%，P值为0.22至0.042，而生长抑制率在-29.7%至-63%之间，P值为0.041至0.022。然而，RNA分析显示，未观察到生物标志物mRNA表达（巢蛋白、NANOG、Sox2、Oct4、CD73、CD90和CD105）有显著变化。这些数据表明，尽管只有高浓度具有统计学意义，但所有浓度的Endocyn均抑制了牙髓干细胞的活力和生长。此外，Endocyn的施用并未改变牙髓干细胞生物标志物的表达，这是以前未观察到或报道过的新颖且重要的发现，可能有助于制定治疗活髓组织的临床决策方案和方法。
