Xia Ming, Li Yifu, Liu Yu, Dong Zheng, Liu Hong
Department of Nephrology, Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Center for Medical Research, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Biomolecules. 2025 Jan 29;15(2):191. doi: 10.3390/biom15020191.
IgA nephropathy (IgAN) is currently the most common primary glomerular disease worldwide, and early diagnosis and intervention contribute significantly to improving outcomes and reducing the incidence of renal failure. The pathogenesis of IgAN remains incompletely understood. In recent years, the rapid development of single-cell RNA-sequencing (scRNA-seq) technology has provided the high-resolution and rich data necessary to elucidate disease characteristics and enabled the analysis of complex interactions between individual cells and cell types. The application of scRNA-seq in IgAN successfully revealed the landscape of immunological features, including peripheral blood B-cell and Th-cell activation, cytotoxic T-cell depletion, and renal infiltrating cell subtypes, as well as the crucial role of mesangial cells and endothelial cells in the early stage of kidney injury, and also revealed the extensive intercellular interactions between renal cells. Here, we discuss the research progress of scRNA-seq technology in IgAN. These scRNA-seq profiles help us to understand the complex molecular mechanisms of IgAN and develop biomarkers and specific therapeutic strategies.
IgA 肾病（IgAN）是目前全球最常见的原发性肾小球疾病，早期诊断和干预对于改善预后及降低肾衰竭发生率具有重要意义。IgAN 的发病机制仍未完全明确。近年来，单细胞 RNA 测序（scRNA-seq）技术的迅速发展提供了阐明疾病特征所需的高分辨率和丰富数据，并能够分析单个细胞及细胞类型之间的复杂相互作用。scRNA-seq 在 IgAN 中的应用成功揭示了免疫特征图谱，包括外周血 B 细胞和 Th 细胞活化、细胞毒性 T 细胞耗竭以及肾浸润细胞亚型，还揭示了系膜细胞和内皮细胞在肾损伤早期的关键作用，同时也揭示了肾细胞之间广泛的细胞间相互作用。在此，我们讨论 scRNA-seq 技术在 IgAN 中的研究进展。这些 scRNA-seq 图谱有助于我们理解 IgAN 的复杂分子机制，并开发生物标志物和特定治疗策略。
