The Detection of Extensively Drug-Resistant Strains Harboring Both VIM-4 and VIM-75 Metallo-β-Lactamases from Patients in Germany.

is a well-known opportunistic pathogen predominantly associated with urinary tract infections. It exhibits natural resistance to multiple antibiotics, including last-resort options like colistin. The emergence and spread of multidrug-resistant isolates, including those producing ESBLs, AmpC cephalosporinases, and carbapenemases, are now more frequently reported. The most common carbapenemase types found in are KPC-2, IMP, VIM, NDM, and OXA-48. We sequenced the genomes of three carbapenem-resistant isolates harboring both and from Germany using both short-read and long-read sequencing techniques. We found that the isolates were only distantly related genetically. Both and genes were located on a class I integron, which in two cases was located on the chromosome and in one case on a plasmid. This is the first report on the complete genomes of strains harboring a rare genetic element encoding both and . Our results emphasize a key role for class 1 integrons in the transmission of VIM carbapenemases in .