Niepold F, Conrad R, Schlegel H G
Antonie Van Leeuwenhoek. 1979;45(3):485-97. doi: 10.1007/BF00443286.
The efficiency of extraction of hydrogen bacteria from soil for plate counting was evaluated by using pure cultures adsorbed to sterilized soil. The utilization of model materials which interact with bacteria by adhesive, capillary or electrostatic forces and the use of extraction fluids with buffering, detergent or chelating activity demonstrated the major importance of capillary forces for the retention of hydrogen bacteria. Utilization of Tris buffer (pH 7.5) as extraction fluid and separation of extracted bacteria from soil particles by sedimentation for 15 min resulted in the highest recovery. A second extraction step including sonication did not increase the efficiency. The extraction efficiency of 8 different strains of hydrogen bacteria adsorbed to 3 different soils demonstrated a high degree of variation wih respect to bacterial strains, but not to soil types. The recovery was inversely related to cell parameters such as size, motility and slime formation.
通过使用吸附在无菌土壤上的纯培养物，评估了从土壤中提取氢细菌用于平板计数的效率。利用通过粘附、毛细管或静电力与细菌相互作用的模型材料，以及使用具有缓冲、去污或螯合活性的提取液，证明了毛细管力对氢细菌保留的重要性。使用Tris缓冲液（pH 7.5）作为提取液，并通过沉降15分钟从土壤颗粒中分离提取的细菌，回收率最高。包括超声处理的第二步提取并没有提高效率。吸附在3种不同土壤上的8种不同氢细菌菌株的提取效率在菌株方面表现出高度差异，但在土壤类型方面没有差异。回收率与细胞参数如大小、运动性和黏液形成呈负相关。
