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关于“变形杆菌”L型的一些生化和血清学特性以及对抗生素敏感性的研究(作者译)

[Researches on some biochemical and serological properties and on the sensitivity to antibiotics of L-forms of "Proteus" (author's transl)].

作者信息

Barbuti S, Montagna M T, Quarto M

出版信息

Ann Sclavo. 1979 Jul-Aug;21(4):456-67.

PMID:400087
Abstract

The possibility to keep some biochemical reactions of the parent strains (urease-positive, glucose fermentation, phenylalanine-deaminase-positive, H2S but not indol production) was demonstrated in 5 L-forms, obtained from as many strains of Pr. mirabilis and in 1 L-form, isolated from a vaginal secretion and identified as belonging to the same species. The indirect hemagglutination technique, made by the sonicated antigen in 3 of the 6 L-forms with Proteus OXK antiserum, resulted positive in titers varying from 1:128 to 1:1024. Crossed tests made with antisera for different bacterial species (e. coli, Shigella, klebsiella, ecc.) and of Mycoplasma (M. hominis, M. orale, M. salivarium, M. fermentans, M. arthritidis) put in evidence aspecific reactions only in 1.3% of the bacterial antisera. On the contrary, all 5 antisera for Mycoplasma were able to agglutinate the sensitized erythrocytes at titers quite analogous to that of the homologous antiserum. The sensitivities to various antibiotics of the 6 L-forms and the parent strains has been determined. All of L-forms were more resistent to the tetracycline than L-forms of other bacterial species. On the basis of te results got by biochemical and serological tests, we confirm the necessity to make use of both the groups of tests, in order to identify the L-forms of recent isolation.

摘要

从同样多的奇异变形杆菌菌株中获得的5种L型细菌以及从一份阴道分泌物中分离出并鉴定为属于同一物种的1种L型细菌,显示出保留亲代菌株某些生化反应的可能性(脲酶阳性、葡萄糖发酵、苯丙氨酸脱氨酶阳性、产生H2S但不产生吲哚)。用变形杆菌OXK抗血清对6种L型细菌中的3种进行超声处理抗原的间接血凝技术检测,效价从1:128到1:1024不等,结果呈阳性。用针对不同细菌种类(大肠杆菌、志贺氏菌、克雷伯氏菌等)和支原体(人型支原体、口腔支原体、唾液支原体、发酵支原体、关节炎支原体)的抗血清进行交叉试验,结果仅在1.3%的细菌抗血清中出现非特异性反应。相反,所有5种支原体抗血清都能够以与同源抗血清相当的效价凝集致敏红细胞。已经测定了6种L型细菌和亲代菌株对各种抗生素的敏感性。所有L型细菌对四环素的耐药性都比其他细菌种类的L型细菌更强。根据生化和血清学试验结果,我们确认有必要同时使用这两组试验,以便鉴定新分离的L型细菌。

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