Li Qing, Jiang Jihui, Cheng Haoyang, Zhang Bin, Zhang Yang, Hu Jian
College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 225127, China.
Key Laboratory of Saline-Alkali Soil Reclamation and Utilization in Coastal Areas, the Ministry of Agriculture and Rural Affairs of China, Yangzhou University, Yangzhou, 225127, China.
World J Microbiol Biotechnol. 2025 Feb 27;41(3):76. doi: 10.1007/s11274-025-04284-z.
In this study, the root-associated bacterial communities of salt-tolerant and salt-sensitive rice grown in coastal saline-alkali soils were characterized at three major growth stages (jointing, heading and maturity) using Illumina MiSeq sequencing. The results showed that the growth stage had a stronger influence on endophytic bacterial diversity than the genotype, with diversity decreasing as growth progressed. However, the rhizospheric bacterial diversity was minimally affected by both growth stage and genotype. The variation in both rhizospheric and endophytic bacterial communities was primarily driven by the growth stages, but was also influenced by the genotype. Interestingly, there were distinct differences in changes in taxon abundance between the rhizospheric and endophytic bacterial communities, suggesting that the assembly mechanisms of these communities may differ, particularly in salt-sensitive rice. Additionally, certain genera were found to be enriched in the rhizosphere and endosphere compared to the bulk soil, and this enrichment varied depending on the growth stage and genotype. Notably, the dominant bacterium Clostridium was consistently enriched in the endosphere of salt-tolerant rice throughout all growth stages. This genus was also found to be more abundant overall in the rhizosphere and endosphere of salt-tolerant rice compared to salt-sensitive rice. These findings provide a deeper understanding of the co-evolution between rice and its associated microbes, and offer valuable insights for the isolation and application of beneficial root-associated bacteria in coastal saline-alkali soils.
在本研究中，利用Illumina MiSeq测序技术，对生长在沿海盐碱地的耐盐和盐敏感水稻在三个主要生长阶段（拔节期、抽穗期和成熟期）的根际相关细菌群落进行了表征。结果表明，生长阶段对内生细菌多样性的影响比基因型更强，随着生长进程多样性降低。然而，根际细菌多样性受生长阶段和基因型的影响最小。根际和内生细菌群落的变化主要由生长阶段驱动，但也受基因型影响。有趣的是，根际和内生细菌群落在分类单元丰度变化上存在明显差异，这表明这些群落的组装机制可能不同，尤其是在盐敏感水稻中。此外，与根际土壤相比，某些属在根际和根内被发现有富集，且这种富集因生长阶段和基因型而异。值得注意的是，优势细菌梭菌在耐盐水稻的整个生长阶段都始终在根内富集。与盐敏感水稻相比，该属在耐盐水稻的根际和根内总体上也更为丰富。这些发现为深入了解水稻与其相关微生物之间的共同进化提供了依据，并为在沿海盐碱地中分离和应用有益的根际相关细菌提供了有价值的见解。
