Karagoz Abdurrahman, Savran Tahir, Yilmaz Ibrahim
Department of Chemistry, Kamil Ozdag Science Faculty, Karamanoglu Mehmetbey University, 70100, Karaman, Türkiye.
Department of Chemistry and Chemical Processing Technologies, Vocational School of Technical Sciences, Karamanoglu Mehmetbey University, 70100, Karaman, Türkiye.
J Fluoresc. 2025 Feb 26. doi: 10.1007/s10895-025-04212-2.
A coumarin based fluorescent probe (E)-N'-((7-hydroxy-2-oxo-2H-chromon-3-yl)methylene)furan-2-carbohydrazide (CFHZ) was synthesized for the detection of Fe and its characterizations were carried out using spectroscopic methods such as FT-IR, mass spectrometryH-NMR, C-NMR. The novel probe CFHZ showed a highly selective and sensitive "turn-off" response to Fe ion without any interference from other analytes. Strong fuorescence quenching phenomena of the CFHZ were observed in EtOH:HO (99/1, v/v) detection system (λ = 470 nm) upon the additions of Fe. The binding stoichiometry between CFHZ and Fe was determined by Job's method, FT-IR and MALDI TOF-MS and found to be 2:1. Also, the binding constant was determined to be 1.82 × 10 M and the limits of detection for the analysis of Fe was measured as 25.7 nM. Besides, experimental applications were carried out for real-time monitoring of Fe in water samples using developed sensor. Additionally, fluorescence imaging experiments for Fe detection of CFHZ probe on test papers were successfully performed.
合成了一种基于香豆素的荧光探针（E）-N'-（（7-羟基-2-氧代-2H-色酮-3-基）亚甲基）呋喃-2-碳酰肼（CFHZ）用于检测铁，并使用傅里叶变换红外光谱（FT-IR）、质谱、氢核磁共振（H-NMR）、碳核磁共振（C-NMR）等光谱方法对其进行了表征。新型探针CFHZ对铁离子表现出高度选择性和灵敏的“关断”响应，不受其他分析物的干扰。在乙醇：水（99/1，v/v）检测体系（λ = 470 nm）中，加入铁后观察到CFHZ强烈的荧光猝灭现象。通过乔布氏法、FT-IR和基质辅助激光解吸电离飞行时间质谱（MALDI TOF-MS）确定CFHZ与铁之间的结合化学计量比为2:1。此外，结合常数测定为1.82×10 M，铁分析的检测限测定为25.7 nM。此外，使用所开发的传感器对水样中的铁进行了实时监测的实验应用。此外，还成功地进行了CFHZ探针在试纸上检测铁的荧光成像实验。
