Efficient Plant Growth-Promoting (PGP) Native -Formulated Consortia Mode and Assessed Shelf Life Using Low-Cost Dynamic Media.

Various isolates were collected from the microbial germplasm, including KAC3 (PP550146), KAC4 (PP177363), KAC6 (PP177364), KAC8 (PP177365), KAC10 (PP177366), KAC11(PP177366), and KAC12 (PP177389). Three consortia formulations were then selected based on their compatibility with plant growth-promoting rhizobacteria (PGPR) properties under laboratory conditions: C consortium (KAC3, KAC10 and KAC11), C (KAC4, KAC8 and KAC10), and C (KAC11 and KAC12). In this study, we explored the use of locally available, cost-effective carrier materials, such as wood ash (NPK ratio: 1.72:0.86:3.6), charcoal (NPK ratio: 0.93:23.68:0.8), eggshell powder, a surfactant agent (SDS + CTAB), and vermi-compost (NPK ratio: 3.5:0.71:0.6). The CFU counts of isolates in the consortia were calculated over six months at different temperatures (28 and 35 ± 2°C) using these low-cost materials. The C consortium showed the highest stability in maintaining viable populations, whereas C and C showed the best performance in the combined-ingredient carrier material. The efficacy of C was monitored using an IoT device and further validated under different temperature conditions for six months. The data showed that temperature variation affected growth, with an optimal viability at 28 ± 2°C. Moreover, increases in temperature led to higher CO levels and decreased moisture and humidity, yet the population remained stable. This highly efficient C consortium formulation is a promising eco-friendly bioinoculant for sustainable agriculture.