Bernardi G, Olofsson B, Filipski J, Zerial M, Salinas J, Cuny G, Meunier-Rotival M, Rodier F
Science. 1985 May 24;228(4702):953-8. doi: 10.1126/science.4001930.
Most of the nuclear genome of warm-blooded vertebrates is a mosaic of very long (much greater than 200 kilobases) DNA segments, the isochores; these isochores are fairly homogeneous in base composition and belong to a small number of major classes distinguished by differences in guanine-cytosine (GC) content. The families of DNA molecules derived from such classes can be separated and used to study the genome distribution of any sequence which can be probed. This approach has revealed (i) that the distribution of genes, integrated viral sequences, and interspersed repeats is highly nonuniform in the genome, and (ii) that the base composition and ratio of CpG to GpC in both coding and noncoding sequences, as well as codon usage, mainly depend on the GC content of the isochores harboring the sequences. The compositional compartmentalization of the genome of warm-blooded vertebrates is discussed with respect to its evolutionary origin, its causes, and its effects on chromosome structure and function.
温血脊椎动物的大多数核基因组是由非常长（远大于200千碱基）的DNA片段即等密度区带构成的镶嵌体；这些等密度区带在碱基组成上相当均匀，属于少数几个以鸟嘌呤 - 胞嘧啶（GC）含量差异区分的主要类别。源自此类别的DNA分子家族可以被分离出来，并用于研究任何可被探测序列的基因组分布。这种方法揭示了：（i）基因、整合病毒序列和散布重复序列在基因组中的分布高度不均匀；（ii）编码和非编码序列中的碱基组成以及CpG与GpC的比例，还有密码子使用情况，主要取决于包含这些序列的等密度区带的GC含量。本文讨论了温血脊椎动物基因组的组成分区在其进化起源、成因及其对染色体结构和功能的影响方面的情况。
