Robinson C P, Baskin S I, Visnich N, Franz D R
Toxicology. 1985 Apr;35(1):59-72. doi: 10.1016/0300-483x(85)90132-5.
Effects of sodium cyanide on isolated strips of rabbit, dog, and ferret aorta were determined. In the rabbit aorta strip, cumulatively added cyanide caused small contractions beginning at approximately 10(-11) M cyanide and reaching a maximum response at 10(-5) M. A concentration of cyanide between 10(-5) M and 10(-3) M produced relaxation. When cyanide was cumulatively added to norepinephrine (NE)-contracted rabbit aorta strips, no contractions were observed. Cyanide concentrations above 10(-5) M produced relaxation in the NE-contracted vessels. Sensitivity of the aorta strips to NE differed among the species examined. The ED50 for contractions in the dog and ferret aorta was 4 X 10(-4) M and in the rabbit was 5 X 10(-6) M. Pretreatment with cyanide in concentrations up to 10(-2) M did not reduce contractions of dog aorta to NE, although 10(-2) M cyanide abolished contractions of rabbit aorta to NE and reversed NE-contractions of ferret aorta to relaxation. The antagonism of cyanide for NE-induced contractions was completely reversible with cyanide concentrations up to 10(-3) M. Cyanide pretreatment of strips of aorta increased the rate of contraction to NE. A concentration of 10(-2) M cyanide caused small contractions of aorta strips from each species. Thus, cyanide exerts dose and species dependent responses on vascular smooth muscle.
测定了氰化钠对兔、犬和雪貂离体主动脉条的作用。在兔主动脉条中，累积添加氰化钠会引起小幅度收缩，从大约10⁻¹¹ M氰化钠开始，在10⁻⁵ M时达到最大反应。氰化钠浓度在10⁻⁵ M至10⁻³ M之间时会产生舒张作用。当将氰化钠累积添加到去甲肾上腺素（NE）收缩的兔主动脉条时，未观察到收缩。高于10⁻⁵ M的氰化钠浓度会使NE收缩的血管产生舒张。在所研究的物种中，主动脉条对NE的敏感性有所不同。犬和雪貂主动脉收缩的半数有效剂量（ED50）为4×10⁻⁴ M，兔为5×10⁻⁶ M。用浓度高达10⁻² M的氰化钠预处理并未降低犬主动脉对NE的收缩，尽管10⁻² M氰化钠消除了兔主动脉对NE的收缩，并使雪貂主动脉的NE收缩转为舒张。氰化钠对NE诱导收缩的拮抗作用在氰化钠浓度高达10⁻³ M时是完全可逆的。用氰化钠预处理主动脉条会增加对NE的收缩速率。10⁻² M的氰化钠浓度会引起每个物种的主动脉条小幅收缩。因此，氰化钠对血管平滑肌产生剂量和物种依赖性反应。
