Setyono Eunike Sawitning Ayu, Rogers Nicole Katarina, Hofmann Anita, Lickert Heiko, Burtscher Ingo
Institute of Diabetes and Regeneration Research, Helmholtz Munich, 85764 Neuherberg, Germany; Technical University Munich 81675 Munich, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany.
Institute of Diabetes and Regeneration Research, Helmholtz Munich, 85764 Neuherberg, Germany.
Stem Cell Res. 2025 Apr;84:103685. doi: 10.1016/j.scr.2025.103685. Epub 2025 Feb 15.
Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.
胰岛由几种协同工作的不同内分泌细胞类型组成。除了原代胰岛外，干细胞衍生的胰岛可用作替代研究和疾病模型。在这里，我们通过CRISPR/Cas9介导的mCherry插入到先前发表的ARX-CFP hiPSC系中C肽的C末端，构建了ARX-T2A-H2B-CFP×C-PEP-mCherry-hiPSC双报告基因系。该报告基因系能够在整个分化过程中对干细胞衍生的胰腺α细胞和β细胞进行实时监测。
