Dubińska-Magiera Magda, Brania Marta Julianna, Kolenda Krzysztof, Haczkiewicz-Leśniak Katarzyna, Daczewska Małgorzata, Migocka-Patrzałek Marta
Department of Animal Developmental Biology, University of Wrocław, Sienkiewicza 21, 50-335, Wrocław, Poland.
Department of Evolutionary Biology and Conservation of Vertebrates, University of Wrocław, Sienkiewicza 21, 50-335, Wrocław, Poland.
Protoplasma. 2025 Mar 1. doi: 10.1007/s00709-025-02047-z.
Extant amphibians are divided into three distinct lineages: Gymnophiona, Urodela, and Anura. Owing to their diversity, they are widely used in research concerning, among others, tissue regeneration. Here, we describe larval myotomal myogenesis in the Chinese fire-bellied newt, Hypselotriton orientalis. Morphological, ultrastructural, cytochemical, and immunohistochemical analyses showed that trunk muscles grow through hypertrophy and hyperplasia mediated by the expression of typical myogenic factors, such as Pax7, from muscle progenitor cells. Our findings also suggest a third alternative mechanism for muscle growth involving the muscle splitting or fragmentation of preexisting larval myotubes.
蚓螈目、有尾目和无尾目。由于它们的多样性，它们被广泛应用于包括组织再生等方面的研究。在这里，我们描述了东方蝾螈幼体肌节的肌发生过程。形态学、超微结构、细胞化学和免疫组织化学分析表明，躯干肌肉通过肌肉祖细胞中典型的生肌因子（如Pax7）的表达介导的肥大和增生而生长。我们的研究结果还提出了肌肉生长的第三种替代机制，即涉及先前存在的幼体肌管的肌肉分裂或碎片化。
