Hashim Kadhim K, Al-Rashidy Asmaa A Mohammed, Humeidy Israa T
Al-Qasim Green University, Hillah, Babylon, 51013, Iraq.
College of Pure Science, Tikrit University, Tikrit, Iraq.
J Fluoresc. 2025 Mar 1. doi: 10.1007/s10895-025-04168-3.
Quantum yield (QY) is a fundamental parameter in luminescence study. This paper introduces a novel SAFE method for determining the fluorescence quantum yield of 5-(2-(dimethylamino)-6-methoxypyrimidin-4-yl)-furan-2-carbaldehyde, which was synthesized from pyrimidine derivatives through the Suzuki cross-coupling reaction. A key contribution of this study is integrating measuring tools into a single device, as opposed to using two separate devices typically found in commercial applications. The study utilizes a commercial fluorescence spectrometer capable of recording absorption and emission spectra simultaneously, ensuring precise and efficient measurements. The fluorescence quantum yields of anthracene, quinine sulfate, tyrosine, and the synthesized pharmacological compound were investigated. Quinine sulfate (QS), dissolved in 0.05 M sulfuric acid, served as the reference standard on a traceable fluorometer. Measurements were conducted using laboratory-designed quantum yield measurement software based on the SAFE method, yielding results with high accuracy relative to standard values. The quantum yields for tyrosine in water, anthracene, and 5-(2-(dimethylamino)-6-methoxypyrimidin-4-yl)-furan-2-carbaldehyde in ethanol were determined at specific excitation wavelengths. For the first set of excitation wavelengths (λ₁) at 270, 330, and 385 nm, the QY values were found to be 13.089, 28.51, and 27.126, respectively. In a second set of excitation wavelengths (λ₂) at 273, 335, and 397 nm, the corresponding QY values were determined to be 13.091, 29.153, and 27.354, with quinine sulfate serving as the reference standard at λ₁ (299 nm) and λ₂ (310 nm). These findings demonstrate the effectiveness of the SAFE method in accurately determining fluorescence quantum yields, offering significant utility for characterizing luminescent compounds and evaluating pharmacological substances.
量子产率（QY）是发光研究中的一个基本参数。本文介绍了一种新颖的SAFE方法，用于测定5-(2-(二甲基氨基)-6-甲氧基嘧啶-4-基)-呋喃-2-甲醛的荧光量子产率，该化合物是通过嘧啶衍生物经铃木交叉偶联反应合成的。本研究的一个关键贡献是将测量工具集成到单个设备中，这与商业应用中通常使用的两个单独设备不同。该研究利用了一台能够同时记录吸收光谱和发射光谱的商用荧光光谱仪，确保了精确而高效的测量。研究了蒽、硫酸奎宁、酪氨酸以及合成的药理化合物的荧光量子产率。溶解于0.05 M硫酸中的硫酸奎宁（QS）在可溯源的荧光计上用作参考标准。使用基于SAFE方法的实验室设计的量子产率测量软件进行测量，相对于标准值，得到的结果具有很高的准确性。在特定激发波长下测定了水中酪氨酸、蒽以及乙醇中5-(2-(二甲基氨基)-6-甲氧基嘧啶-4-基)-呋喃-2-甲醛的量子产率。对于第一组激发波长（λ₁），分别为270、330和385 nm，发现量子产率值分别为13.089、28.51和27.126。在第二组激发波长（λ₂），分别为273、335和397 nm，相应的量子产率值测定为13.091、29.153和27.354，硫酸奎宁在λ₁（299 nm）和λ₂（310 nm）用作参考标准。这些发现证明了SAFE方法在准确测定荧光量子产率方面的有效性，为表征发光化合物和评估药理物质提供了重要的实用价值。
