Kumar Rajesh, Dhar Irra, Sharma Madan Mohan
Department of Biosciences, Manipal University Jaipur, Dehmi Kalan, Near JVK Toll Plaza, Jaipur-Ajmer Expressway, Jaipur 303007, Rajasthan, India.
ACS Omega. 2025 Feb 14;10(7):7181-7193. doi: 10.1021/acsomega.4c10628. eCollection 2025 Feb 25.
This research examines the environmentally benign manufacture of zinc oxide nanoparticles employing a crude extract from leaves as a capping and reducing agent. The considerable peak of synthesized zinc oxide nanoparticles (ZnO NPs) was observed at 335 nm, and the functional groups of plant active metabolites to reduce zinc and evaluate shape and elemental compositions were analyzed using UV-vis spectroscopy, FT-IR, SEM, and EDX analysis, respectively. The average size of synthesized zinc oxide nanoparticles (27.26 nm) was validated by XRD using the Debye-Scherrer's equation. Zinc oxide nanoparticles were assessed for their efficiency in seed priming, photocatalytic degradation, antibacterial activity, and antioxidant abilities. The biosynthesized zinc oxide nanoparticles were utilized in seed priming, significantly enhancing germination rate (90%), shoot length (5.46 cm), and root length (15.13 cm) at a concentration of 150 ppm in comparison to control. Further, the effect of methyl orange (MO) and methylene blue (MB) dyes on % seed germination and plant growth of hybrid pearl millet was studied . MO and MB had shown approximately 15 and 46% reduction in seed germination % in comparison to control. Additionally, zinc oxide nanoparticles had shown remarkable photocatalytic degradation of 94.45% against methylene blue and 85.99% against methyl orange. Zinc oxide nanoparticles were also effective against and bacteria, with zones of inhibition of 0.45 and 0.35 cm at a 100 mg/mL concentration. Furthermore, zinc nanoparticles observed higher antioxidant activity against DPPH at 80 μg/mL. The present finding highlights the potential of biosynthesized zinc oxide nanoparticles as a sustainable approach to agriculture, environmental remediation, and biological sciences.
本研究考察了以树叶粗提物作为封端和还原剂对氧化锌纳米颗粒进行环境友好型制备。在335nm处观察到合成的氧化锌纳米颗粒（ZnO NPs）有明显的峰，分别使用紫外可见光谱、傅里叶变换红外光谱、扫描电子显微镜和能谱分析对植物活性代谢产物还原锌以及评估形状和元素组成的官能团进行了分析。利用德拜-谢乐方程通过X射线衍射验证了合成的氧化锌纳米颗粒的平均尺寸（27.26nm）。对氧化锌纳米颗粒在种子引发、光催化降解、抗菌活性和抗氧化能力方面的效率进行了评估。生物合成的氧化锌纳米颗粒用于种子引发，与对照相比，在150ppm浓度下显著提高了发芽率（90%）、苗长（5.46cm）和根长（15.13cm）。此外，研究了甲基橙（MO）和亚甲基蓝（MB）染料对杂交珍珠粟种子发芽率和植株生长的影响。与对照相比，MO和MB使种子发芽率分别降低了约15%和46%。此外，氧化锌纳米颗粒对亚甲基蓝和甲基橙分别表现出94.45%和85.99%的显著光催化降解率。氧化锌纳米颗粒对 和 细菌也有效，在100mg/mL浓度下抑菌圈分别为0.45cm和0.35cm。此外，在80μg/mL时观察到锌纳米颗粒对二苯基苦味酰基自由基具有更高的抗氧化活性。本研究结果突出了生物合成的氧化锌纳米颗粒在农业、环境修复和生物科学领域作为一种可持续方法的潜力。
